PanB over-representation as part of pyrazinamide action: a proteomic insight
| Autor: | Isabella Letícia Esteves Barros, Eduardo Jorge Pilau, Luciana Dias Ghiraldi-Lopes, Glaucia Sayuri Arita, Paula Aline Zanetti Campanerut-Sá, Vera Lúcia Dias Siqueira, Jean Eduardo Meneguello, Rosilene Fressatti Cardoso, Katiany Rizzieri Caleffi Ferracioli, João Vitor de Oliveira Silva, Regiane Bertin de Lima Scodro |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Microbiology (medical) Antituberculosis drug Bacillus (shape) Tuberculosis biology Chemistry Drug target Antitubercular Agents Microbial Sensitivity Tests Mycobacterium tuberculosis Pyrazinamide biology.organism_classification medicine.disease Microbiology Over representation Drug Resistance Bacterial medicine Humans medicine.drug |
| Zdroj: | Future Microbiology. 16:1303-1308 |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb-2020-0307 |
| Popis: | Background: Pyrazinamide (PZA) represents a milestone as a first-line antituberculosis drug due to its sterilizing activity against Mycobacterium tuberculosis. Materials & Methods: The protein changes induced by subinhibitory PZA exposure of M. tuberculosis in acidic pH were evaluated by a proteomic approach. Results: Among the 1059 M. tuberculosis proteins identified, the specific acidification in the culture medium induced the over-representation of MurF (Rv2157c), and its under-representation was induced by 12 h of PZA exposure. PanB (Rv2225) was over-represented at 24 h of PZA exposure. Conclusion: The authors highlight the over-representation of PanB in M. tuberculosis correlates of PZA action in acidic pH, reinforcing the role of the pantothenate pathway as a bacillus drug target to be explored. |
| Databáze: | OpenAIRE |
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