High histone variant H3.3 content in mouse prospermatogonia suggests a role in epigenetic reformatting
| Autor: | Michelle C. W. Tang, Jeffrey R. Mann, Steve Binos, Eng Kok Ong, Lee H. Wong |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Blotting Western Gene Expression Mice Transgenic Biology Gas Chromatography-Mass Spectrometry Epigenesis Genetic Histones Mice Histone H3 Histone H2A Histone methylation Genetics Animals Histone code Epigenetics Cancer epigenetics Genetics (clinical) Epigenomics DNA Methylation Chromatin Assembly and Disassembly Spermatogonia Nucleosomes Histone methyltransferase Female |
| Zdroj: | Chromosoma. 123:587-595 |
| ISSN: | 1432-0886 0009-5915 |
| DOI: | 10.1007/s00412-014-0475-8 |
| Popis: | Histone variants can incorporate into the nucleosome outside of S-phase. Some are known to play important roles in mammalian germ cell development, this cell lineage being characterized by long phases of quiescence, a protracted meiotic phase, and genome-wide epigenetic reformatting events. The best known example of such an event is the global-scale erasure of DNA methylation in sexually indifferent primordial germ cells, then its re-establishment in fetal prospermatogonia and growing oocytes. Histone H3 and its post-translationally modified forms provide important waypoints in the establishment of epigenetic states. Using mass spectrometry and immunoblotting, we show that the H3.3 replacement variant is present at an unusually high amount in mouse prospermatogonia at the peak stage of global DNA methylation re-establishment. We speculate that H3.3 facilitates this process through achieving a greater level of accessibility of chromatin modifiers to DNA. |
| Databáze: | OpenAIRE |
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