Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation,and Breast Cancer
| Autor: | Nesrine Gargouch, Imen Fendri, Chantal Pichon, Fatma Elleuch, Ines Karkouch, Luc Picton, Slim Abdelkafi, Christine Gardarin, Olfa Tabbene, Christophe Rihouey, Céline Laroche |
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| Přispěvatelé: | Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Biotechnologies Végétales Appliquées à l'Amélioration des Cultures (Sfax, Tunisie), Université de Sfax - University of Sfax, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3029, Centre de Biotechnologie de Borj Cedria (CBBC), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Sfax, Tournayre, Christophe |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering Cell Survival Pharmaceutical Science Porphyridium marinum Breast Neoplasms Microbial Sensitivity Tests 01 natural sciences Article 03 medical and health sciences Minimum inhibitory concentration Mice antibacterial activity 010608 biotechnology Cell Line Tumor Drug Discovery Microalgae Animals [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Food science Candida albicans lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology 0303 health sciences Molar mass biology Dose-Response Relationship Drug Chemistry Polysaccharides Bacterial Biofilm Biological activity biology.organism_classification 3. Good health Anti-Bacterial Agents high pressure homogenizer lcsh:Biology (General) anti-biofilm activity Biofilms Porphyridium anti-cancer activity exopolysaccharide Composition (visual arts) Female Antibacterial activity |
| Zdroj: | Marine drugs Marine drugs, MDPI, 2020, 19 (2), pp.66. ⟨10.3390/md19020066⟩ Marine Drugs Marine drugs, 2020, 19 (2), pp.66. ⟨10.3390/md19020066⟩ Marine Drugs, Vol 19, Iss 66, p 66 (2021) Volume 19 Issue 2 |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md19020066⟩ |
| Popis: | Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µ g/mL to 1000 µ g/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µ g/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers. |
| Databáze: | OpenAIRE |
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