UPLC/Q-TOF-MS/MS-based metabolomics revealed the lipid-lowering effect of Ilicis Rotundae Cortex on high-fat diet induced hyperlipidemia rats
| Autor: | Shiqin Jiang, Xiang Luo, Bao Yang, Liping Zhu, Zhongxiang Zhao, Qingfeng Ruan, Shenxin Xuan, Hui Cui, Jing Jin |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Taurine China Glyoxylate and dicarboxylate metabolism Blood lipids Hypotaurine Hyperlipidemias Pharmacology Diet High-Fat Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry Drug Discovery Hyperlipidemia medicine Animals Metabolomics Chromatography High Pressure Liquid 030304 developmental biology Hypolipidemic Agents chemistry.chemical_classification 0303 health sciences Fatty Acids Fatty acid Metabolism medicine.disease Lipid Metabolism Lipids Rats chemistry Liver 030220 oncology & carcinogenesis Ketone bodies Metabolome Biomarkers |
| Zdroj: | Journal of ethnopharmacology. 256 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Ilicis Rotundae Cortex (IRC), a Chinese crude drug, has been widely utilized in Guangdong and Guangxi provinces of China to treat or prevent cardiovascular diseases. Aim of study This investigation aims to study the lipid-lowering effect of IRC, as well as the regulating effect on the endogenous metabolites in hyperlipidemia rats. Materials and methods High-fat diet induced hyperlipidemia rats were administrated with different doses of IRC extract (0.5, 1.0 and 2.0 g/kg/day) for 5 weeks. Simvastatin was used as the positive control. Body weight, serum lipid levels and histopathology of liver were evaluated. The metabolic profiles of plasma, urine and cecum content were analyzed using UPLC/Q-TOF-MS/MS-based metabolomics approach coupled with multivariate data analysis. Results The levels of serum TC, TG, LDL-C, AST and ALT were significantly decreased and HDL-C level was increased in IRC treatment groups. The hepatic histomorphology was partially restored. 23, 26 and 15 metabolites in plasma, urine and cecum content were determined as the biological biomarkers, respectively. IRC extract could partially recover the disturbed metabolic pathways of bile acid metabolism, linoleic acid metabolism, arachidonic acid metabolism, taurine and hypotaurine metabolism, glyoxylate and dicarboxylate metabolism, glycerophospholipid metabolism, synthesis and degradation of ketone bodies, sphingolipid metabolism and riboflavin metabolism. Conclusion This study demonstrated that IRC could effectively improve the serum lipids and partially restore the hepatic histomorphology. The underlying metabolic mechanism mainly included improving the metabolism of bile acids, glycerophospholipid, sphingolipid, fatty acid and amino acid. This is the first study on the lipid-lowering effect of IRC from the perspective of metabolomics. |
| Databáze: | OpenAIRE |
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