RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda
| Autor: | Marcos Valério Garcia, Claire Julie Akwongo, Renato Andreotti, Nanteza Ann, Fábio Pereira Leivas Leite, Kokas Ikwap, Rodrigo Casquero Cunha, Emukule Samuel, Margaret Saimo-Kahwa, Francisco Denis Souza Santos |
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| Rok vydání: | 2021 |
| Předmět: |
ra92a protein
Veterinary medicine Immunogen QH301-705.5 immunogenicity Tick ticks Pichia pastoris Ticks Antigen parasitic diseases Biology (General) biology Vaccine Agricultural Sciences Immunogenicity Agriculture biology.organism_classification vaccine Immunization Ra92A protein biology.protein Rhipicephalus microplus Antibody Rhipicephalus appendiculatus General Agricultural and Biological Sciences rhipicephalus appendiculatus |
| Zdroj: | Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37068 Bioscience Journal ; v. 37 (2021): Continuous Publication; e37068 Bioscience journal Universidade Federal de Uberlândia (UFU) instacron:UFU Bioscience Journal, Vol 37, Pp e37068-e37068 (2021) |
| ISSN: | 1981-3163 |
| Popis: | In this study, the recombinant gut protein rRa92A produced in Pichia pastoris yeast cells was used to immunize cattle in two experiments, one in Brazil and the other in Uganda. In both experiments, the animals were intramuscularly (IM) injected with 200 µg of recombinant protein in Brazil on days 0, 30 and 51 and in Uganda on days 0, 30. Blood samples for sera separation were collected from different days in both experiments. These samples were analyzed by ELISAs. In Brazil, ticks collected from the animals during the experimental period were analyzed for biological parameters. At Uganda, blood was collected to assess blood parameters, clinical signs were recorded and adult tick (Rhipicephalus appendiculatus) counts were performed. All animals of the vaccinated groups were shown to produce antibodies, and it was not possible to detect an effect on Rhipicephalus microplus. All the clinical parameters were considered within the normal ranges for both the experimental and control groups in Uganda. Antibody absorbance was elevated after each immunization and remained high until the end of the experiments, remaining low in the control animals. The results of stall test carried out in Brazil using R. microplus tick showed efficacy of 21.95%. The rRa92A immunization trial experiments in Uganda showing a decrease of 55.2% in the number of engorged adult ticks, which was statistically significant (p |
| Databáze: | OpenAIRE |
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