Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation
| Autor: | Luciana Souza, de Aragão-França, Luciana S, Aragão-França, Viviane Costa Junqueira, Rocha, Viviane C J, Rocha, Andre, Cronemberger-Andrade, Fábio Henrique Brasil, da Costa, F H B, Costa, Juliana Fraga, Vasconcelos, José Fernandes, Vasconcelos, Daniel Abensur, Athanazio, Daniela Nascimento, Silva, Emanuelle Souza, Santos, E S, Santos, Cássio Santana, Meira, Cintia Figueiredo, Araujo, C F, Araújo, Jéssica Vieira, Cerqueira, Fabíola, Cardillo, Neuza Maria, Alcântara-Neves, Milena Botelho Pereira, Soares, Lain Carlos, Pontes de Carvalho, Lain C, Pontes-de-Carvalho |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Allergy medicine.medical_treatment Immunology Immunoglobulin E Allergic inflammation 03 medical and health sciences 0302 clinical medicine Immune system Splenocyte medicine Immunology and Allergy biology medicine.diagnostic_test business.industry medicine.disease respiratory tract diseases Transplantation 030104 developmental biology Cytokine Bronchoalveolar lavage biology.protein Erratum business 030215 immunology |
| Zdroj: | Allergy, Asthma & Immunology Research |
| ISSN: | 2092-7363 2092-7355 |
| Popis: | Purpose The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions. |
| Databáze: | OpenAIRE |
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