Clinical application of polymerase chain reaction amplification to diagnosis of herpes virus infection
Autor: | Thomas Ca, Morgan Tm, White Wl, Feldman, Smith Se |
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Rok vydání: | 1994 |
Předmět: |
Viral culture
viruses Gene Amplification Negative control Herpes Simplex Dermatology General Medicine Herpes virus culture Biology Virology Herpes Zoster Polymerase Chain Reaction Sensitivity and Specificity Pathology and Forensic Medicine law.invention chemistry.chemical_compound chemistry Herpes virus law DNA Viral Humans Culture negative Dna viral Polymerase chain reaction DNA |
Zdroj: | The American Journal of dermatopathology. 16(3) |
ISSN: | 0193-1091 |
Popis: | Amplification of viral DNA offers a potentially sensitive and specific method for identifying herpes viruses in pathologic specimens. The purpose of this study is to assess the value of polymerase chain reaction amplification of DNA as a diagnostic test for herpes virus in pathologic specimens. The purpose of this study is to assess for herpes virus infections. We examined 79 paraffin-embedded tissue samples from 43 patients and 55 viral culture samples from 45 patients. Herpes virus DNA in the specimens was amplified by polymerase chain reaction. Using paraffin-embedded tissue on which a diagnosis of herpes virus was made by morphologic criteria, 11 of 19 patients had herpes virus DNA identified by PCR; herpes virus DNA was not identified in any of 35 negative control specimens. Herpes virus DNA was also identified by polymerase chain reaction in all of the positive herpes virus culture specimens. Of 29 culture negative specimens, herpes virus DNA was identified in six. We conclude that polymerase chain reaction is useful to establish or confirm the presence of a herpes virus infection in paraffin-embedded tissue samples and that it is more sensitive than viral culture. |
Databáze: | OpenAIRE |
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