α-Fucosidases with different substrate specificities from two species of Fusarium
| Autor: | Jonathan D. Walton, Janet M. Paper, John S. Scott-Craig, Mareike Bongers, Richard E. Wiemels, Ahmed Faik, David Cavalier, Melissa S. Borrusch |
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| Rok vydání: | 2012 |
| Předmět: |
alpha-L-Fucosidase
Genetics Subfamily Sequence Homology Amino Acid Sequence analysis Molecular Sequence Data Fungal genetics food and beverages Sequence alignment Sequence Analysis DNA General Medicine Biology biology.organism_classification Applied Microbiology and Biotechnology Substrate Specificity Pichia pastoris Microbiology Fusarium Fusarium oxysporum Glycosides DNA Fungal Sequence Alignment Gene Trichoderma reesei Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 97:5371-5380 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share |
| Databáze: | OpenAIRE |
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