Matrix stiffness mechanosensing modulates the expression and distribution of transcription factors in Schwann cells
| Autor: | Rosso, Gonzalo, Wehner, Daniel, Schweitzer, Christine, Möllmert, Stephanie, Sock, Elisabeth, Guck, Jochen, Shahin, Victor |
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| Přispěvatelé: | Universitäts- und Landesbibliothek Münster |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
cell plasticity
integumentary system Short Communication 610 Medicine and health RM1-950 extracellular matrix stiffness mechanosensing nerve regeneration neuron Schwann cell Chemical engineering nervous system Medicine and health TP155-156 ddc:610 Therapeutics. Pharmacology TP248.13-248.65 Biotechnology |
| Zdroj: | Bioengineering & Translational Medicine Bioengineering & Translational Medicine, Vol 7, Iss 1, Pp n/a-n/a (2022) |
| Popis: | After peripheral nerve injury, mature Schwann cells (SCs) de-differentiate and undergo cell reprogramming to convert into a specialized cell repair phenotype that promotes nerve regeneration. Reprogramming of SCs into the repair phenotype is tightly controlled at the genome level and includes downregulation of pro-myelinating genes and activation of nerve repair-associated genes. Nerve injuries induce not only biochemical but also mechanical changes in the tissue architecture which impact SCs. Recently, we showed that SCs mechanically sense the stiffness of the extracellular matrix and that SC mechanosensitivity modulates their morphology and migratory behavior. Here, we explore the expression levels of key transcription factors and myelin-associated genes in SCs, and the outgrowth of primary dorsal root ganglion (DRG) neurites, in response to changes in the stiffness of generated matrices. The selected stiffness range matches the physiological conditions of both utilized cell types as determined in our previous investigations. We find that stiffer matrices induce upregulation of the expression of transcription factors Sox2, Oct6, and Krox20, and concomitantly reduce the expression of the repair-associated transcription factor c-Jun, suggesting a link between SC substrate mechanosensing and gene expression regulation. Likewise, DRG neurite outgrowth correlates with substrate stiffness. The remarkable intrinsic physiological plasticity of SCs, and the mechanosensitivity of SCs and neurites, may be exploited in the design of bioengineered scaffolds that promote nerve regeneration upon injury. Finanziert über die DEAL-Vereinbarung mit Wiley 2019-2022. |
| Databáze: | OpenAIRE |
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