Laser printing of microbial systems: effect of absorbing metal film
| Autor: | M. V. Gorlenko, Vladimir Yusupov, Nikita V. Minaev, E.S. Churbanova, Victor N. Bagratashvili, Boris N. Chichkov, V.S. Cheptsov, L.V. Lysak |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromium Materials science 02 engineering and technology Biosensing Techniques Bacterial growth Applied Microbiology and Biotechnology law.invention Metal 03 medical and health sciences law Titanium Laser printing Bacteria business.industry Lasers 021001 nanoscience & nanotechnology Laser Lift (force) 030104 developmental biology visual_art Printing Three-Dimensional visual_art.visual_art_medium Optoelectronics Printing Gold 0210 nano-technology business Biosensor |
| Zdroj: | Letters in applied microbiology. 67(6) |
| ISSN: | 1472-765X |
| Popis: | Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. SIGNIFICANCE AND IMPACT OF THE STUDY: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems. |
| Databáze: | OpenAIRE |
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