Pluripotent stem cells derived from mouse and human white mature adipocytes

Autor: Kristina I. Boström, Medet Jumabay, Sepideh Heydarkhan-Hagvall, Yucheng Yao, Daniel A. Dumesic, Albert Ly, Raushan Abdmaulen, Laurine J. Shahmirian, Mark R. Cubberly
Rok vydání: 2014
Předmět:
Male
Cellular differentiation
Adipocytes
White
Nude
Adipose
Medical Biotechnology
Myocardial Infarction
Cell Culture Techniques
White
Stem cell marker
Inbred C57BL
Regenerative Medicine
Transgenic
Mice
Adipocytes
Induced pluripotent stem cell
Cells
Cultured
Adult stem cells
Cultured
Stem cell
Stem Cell Research - Induced Pluripotent Stem Cell - Human
Teratoma
General Medicine
Differentiation
Adult stem cell
Pluripotent Stem Cells
Plasticity
Cells
1.1 Normal biological development and functioning
Green Fluorescent Proteins
Clinical Sciences
Mice
Nude
Mice
Transgenic
Germ layer
Biology
Underpinning research
Animals
Humans
Stem Cell Research - Induced Pluripotent Stem Cell
Animal
Myocardium
Cell Biology
Cell Dedifferentiation
Tissue-Specific Progenitor and Stem Cells
Stem Cell Research
Molecular biology
Embryonic stem cell
Mice
Inbred C57BL
Disease Models
Animal
Cell culture
Disease Models
Biochemistry and Cell Biology
Biomarkers
Developmental Biology
Zdroj: Stem cells translational medicine, vol 3, iss 2
Popis: White mature adipocytes give rise to so-called dedifferentiated fat (DFAT) cells that spontaneously undergo multilineage differentiation. In this study, we defined stem cell characteristics of DFAT cells as they are generated from adipocytes and the relationship between these characteristics and lineage differentiation. Both mouse and human DFAT cells, prepared from adipose tissue and lipoaspirate, respectively, showed evidence of pluripotency, with a maximum 5–7 days after adipocyte isolation. The DFAT cells spontaneously formed clusters in culture, which transiently expressed multiple stem cell markers, including stage-specific embryonic antigens, and Sca-1 (mouse) and CD105 (human), as determined by real-time polymerase chain reaction, fluorescence-activated cell sorting, and immunostaining. As the stem cell markers decreased, markers characteristic of the three germ layers and specific lineage differentiation, such as α-fetoprotein (endoderm, hepatic), Neurofilament-66 (ectoderm, neurogenic), and Troponin I (mesoderm, cardiomyogenic), increased. However, no teratoma formation was detected after injection in immunodeficient mice. A novel modification of the adipocyte isolation aimed at ensuring the initial purity of the adipocytes and avoiding ceiling culture allowed isolation of DFAT cells with pluripotent characteristics. Thus, the adipocyte-derived DFAT cells represent a plastic stem cell population that is highly responsive to changes in culture conditions and may benefit cell-based therapies.
Databáze: OpenAIRE
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