Transgenic mice as a model to study the regulation of human transferrin expression in Sertoli cells
| Autor: | Maria-Carla Saleh, Charlotte Lécureuil, Isabelle Fontaine, Mario M. Zakin, Bruno Baron, Florian Guillou |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Genetically modified mouse endocrine system medicine.medical_specialty medicine.medical_treatment Transgene Mice Transgenic Biology Testicle Mice 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Humans Insulin Tissue Distribution Secretion Vitamin A 030304 developmental biology chemistry.chemical_classification 0303 health sciences Sertoli Cells 030219 obstetrics & reproductive medicine Tumor Necrosis Factor-alpha Rehabilitation Transferrin Obstetrics and Gynecology Sertoli cell In vitro Cell biology Drug Combinations medicine.anatomical_structure Endocrinology Cytokine Reproductive Medicine chemistry Follicle Stimulating Hormone |
| Zdroj: | Human Reproduction. 19:1300-1307 |
| ISSN: | 1460-2350 |
| DOI: | 10.1093/humrep/deh297 |
| Popis: | BACKGROUND: Understanding the regulation of proteins secreted by human Sertoli cells is important for identifying the causes of infertility in men. However, experiments with Sertoli cells purified from healthy testes are difficult to perform, for obvious ethical reasons. Therefore, experiments with transgenic mouse models could provide an alternative approach to study the function and regulation of a human gene in Sertoli cells. METHODS: To validate this approach, transgenic mice were generated using phage P1 containing an 80 kbp insert encompassing the complete human transferrin (hTf) gene. The expression pattern of hTf in the mouse background was analysed by isolating Sertoli cells from transgenic mice and comparing the regulation of the human and mouse Tf genes by hormones, retinoids and a cytokine in vitro. RESULTS: The hTf gene in transgenic mice shows a tissue-specific expression pattern that mimics the pattern observed in the human. In Sertoli cell cultures, FSH, insulin, retinol or tumour necrosis factor-a (TNF-a) stimulated hTf secretion, while testosterone alone had no effect. A combination of FSH, insulin, retinol and testosterone or a combination of TNF-a and retinol stimulated hTf secretion, but no additive effect was observed. CONCLUSION: Besides their well-known advantages, transgenic mice seem to be useful models to recapitulate the normal regulation of a human gene. |
| Databáze: | OpenAIRE |
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