Rapid induction of vitamin B12 deficiency in Caenorhabditis elegans cultured in axenic medium
| Autor: | Markandeya Jois, Surafel M. Tegegne, Devin Benheim, Matthew Flavel, Damien L. Callahan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nutrition and Dietetics Endocrinology Diabetes and Metabolism nutritional and metabolic diseases Motility lcsh:TX341-641 Biology biology.organism_classification Quiescence C. elegans Lifespan Axenic Growth Deficiency Vitamin B12 Fertility Cell biology lcsh:Biochemistry 03 medical and health sciences 030104 developmental biology Retarded growth Reduced fertility polycyclic compounds lcsh:QD415-436 B12 deficiency lcsh:Nutrition. Foods and food supply Caenorhabditis elegans Food Science |
| Zdroj: | Journal of Nutrition & Intermediary Metabolism, Vol 13, Iss, Pp 20-25 (2018) Journal of nutrition & intermediary metabolism, 13:20-25 |
| ISSN: | 2352-3859 |
| Popis: | Animal models of B12 deficiency have proven to be difficult due to storage of substantial amounts in the liver and the length of time required to maintain animals on a B12 deficient diet to induce deficiency. Caenorhabditis elegans (C. elegans), due to its short lifespan, has recently emerged as an alternate model to investigate vitamin B12 (B12) deficiency. However, when C. elegans are maintained on bacterial diet, five generations of B12 deficient diet is required before the worms show signs of deficiency. Here we show that C. elegans grown in chemically defined axenic medium without added B12 exhibit signs of deficiency within one generation. Worms grown in deficient media had lower cobalt concentration, retarded growth, reduced fertility, increased motility, reduced quiescence and a shortened lifespan. In conclusion, C. elegans cultured in a defined axenic medium is a suitable and rapid model for studies on B12 deficiency. Keywords: Axenic, C. elegans, Deficiency, Fertility, Growth, Lifespan, Quiescence, Vitamin B12 |
| Databáze: | OpenAIRE |
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