Transcriptional regulation of NOX genes expression in human breast adenocarcinoma MCF-7 cells is modulated by adaptor protein Ruk/CIN85
| Autor: | S. V. komISAreNko, L. B. DroBot, Horak Ir, A. V. Bazalii, Pasi chn yk Gv |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Transcription
Genetic 030204 cardiovascular system & hematology 030226 pharmacology & pharmacy Biochemistry lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Transcriptional regulation Humans lcsh:QD415-436 RNA Messenger RNA Small Interfering PI3K/AKT/mTOR pathway Adaptor Proteins Signal Transducing Regulation of gene expression biology Reverse Transcriptase Polymerase Chain Reaction NADPH Oxidases Signal transducing adaptor protein NOX4 Dual Oxidases Molecular biology Clone Cells Cell biology Gene Expression Regulation Neoplastic Isoenzymes Cell culture NOX1 MCF-7 Cells NADPH Oxidase 1 biology.protein P22phox Reactive Oxygen Species |
| Zdroj: | Ukrainian Biochemical Journal, Vol 88, Iss 1, Pp 119-125 (2016) |
| ISSN: | 2413-5003 2409-4943 |
| Popis: | NADPH oxidases are key components of redox-dependent signaling networks involved in the control of cancer cell proliferation, survival and invasion. The data have been accumulated that demonstrate specific expression patterns and levels of NADPH oxidase homologues (NOXs) and accessory genes in human cancer cell lines and primary tumors as well as modulation of these parameters by extracellular cues. Our previous studies revealed that ROS production by human colorectal adenocarcinoma HT-29 cells is positively correlated with adaptor protein Ruk/CIN85 expression while increased levels of Ruk/CIN85 in weakly invasive human breast adenocarcinoma MC F-7 cells contribute to their malignant phenotype through the constitutive activation of Src/Akt pathway. In this study, to investigate whether overexpression of Ruk/CIN85 in MC F-7 cells can influence transcriptional regulation of NOXs genes, the subclones of MCF-7 cells with different levels of Ruk/CIN85 were screened for NOX1, NOX2, NOX3, NOX4, NOX5, DUOX1 and DUOX2 as well as for regulatory subunit p22Phox mRNA contents by quantitative RT-PCR (qPCR). Systemic multidirectional changes in mRNA levels for NOX1, NOX2, NOX5, DUOX2 and p22Phox were revealed in Ruk/CIN85 overexpressing cells in comparison to control WT cells. Knocking down of Ruk/CIN85 using technology of RNA-interference resulted in the reversion of these changes. Further studies are necessary to elucidate, by which molecular mechanisms Ruk/CIN85 could affect transcriptional regulation of NOXs genes. |
| Databáze: | OpenAIRE |
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