Characterizing Patients with Recurrent Urinary Tract Infections in Vesicoureteral Reflux: A Pilot Study of the Urinary Proteome
| Autor: | Dijana Vitko, Shannon E. DiMartino, John W. Froehlich, Matthew A. Migliozzi, Peter G. Warren, Lily D. Cabour, Stephen A. Kostel, Richard S. Lee, Tanya Logvinenko, Patricia S. Cho |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Proteome Urinary system Pilot Projects Inflammation Urine urologic and male genital diseases Biochemistry Vesicoureteral reflux Gastroenterology Analytical Chemistry Diagnostic modalities 03 medical and health sciences Recurrence Internal medicine Humans Medicine Molecular Biology 030304 developmental biology Vesico-Ureteral Reflux 0303 health sciences Spectral counting business.industry Research 030302 biochemistry & molecular biology Acute-phase protein medicine.disease female genital diseases and pregnancy complications Urinary Tract Infections Cohort Female medicine.symptom Peptides business |
| Zdroj: | Mol Cell Proteomics |
| ISSN: | 1535-9476 |
| DOI: | 10.1074/mcp.ra119.001873 |
| Popis: | Recurrent urinary tract infections (UTIs) pose a significant burden on the health care system. Underlying mechanisms predisposing children to UTIs and associated changes in the urinary proteome are not well understood. We aimed to investigate the urinary proteome of a subset of children who have vesicoureteral reflux (VUR) and recurrent UTIs because of their risk of developing infection-related renal damage. Improving diagnostic modalities to identify UTI risk factors would significantly alter the clinical management of children with VUR. We profiled the urinary proteomes of 22 VUR patients with low grade VUR (1–3 out of 5), a history of recurrent UTIs, and renal scarring, comparing them to those obtained from 22 age-matched controls. Urinary proteins were analyzed by mass spectrometry followed by protein quantitation based on spectral counting. Of the 2,551 proteins identified across both cohorts, 964 were robustly quantified, as defined by meeting criteria with spectral count (SC) ≥2 in at least 7 patients in either VUR or control cohort. Eighty proteins had differential expression between the two cohorts, with 44 proteins significantly up-regulated and 36 downregulated (q |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|