Hypoglycemia after Bariatric Surgery in Mice and Optimal Dosage and Efficacy of Glucose Supplementation
| Autor: | Kristin N Grimsrud, Kevin C K Lloyd, Zoe Y Hsi, Leslie A Stewart |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty 040301 veterinary sciences Injections Subcutaneous Gastric Bypass Hypoglycemia Inbred C57BL Medical and Health Sciences General Biochemistry Genetics and Molecular Biology Injections 0403 veterinary science Subcutaneous injection Mice Pharmacokinetics Pain assessment Diabetes mellitus Medicine Animals Obesity Veterinary Sciences Adverse effect Metabolic and endocrine Original Research General Veterinary Agricultural and Veterinary Sciences business.industry Animal Subcutaneous Prevention Diabetes 04 agricultural and veterinary sciences Biological Sciences medicine.disease Pathophysiology Surgery Mice Inbred C57BL Disease Models Animal Editorial Glucose Disease Models Female business |
| Zdroj: | Comp Med Comparative medicine, vol 70, iss 2 |
| Popis: | The Roux-en-Y Gastric Bypass (RYGB) mouse model is a vital tool for studying the pathophysiology of bariatric surgery and contributes greatly to research on obesity and diabetes. However, complications including postsurgical hypoglycemia can have profoundly negative effects. Unlike in humans, blood glucose (BG) is not typically managed in postoperative rodents, despite their critical role as translational models; without this management, rodents can experience hypoglycemia, potentially impairing wound healing, decreasing survivability, complicating interpretation of research data, and limiting translational utility. In this project, we sought to identify an optimal method for minimally invasive administration of dextrose in C57BL/6N (n = 16; 8 male, 8 female) mice. To do so, we characterized BG pharmacokinetic profiles after subcutaneous and oral–transmucosal (OTM) administration of dextrose. Compared with OTM dosage, the subcutaneous route provided more consistent and reliable delivery of glucose and did not cause significant adverse reactions. We then evaluated the frequency of hypoglycemic events after RYGB in C57BL/6N mice (n = 16; 8 male, 8 female) and the effects of subcutaneous dextrose supplementation on morbidity and mortality. BG measurement and behavioral pain assessment (grimace test) were performed for 3 d after surgery. Hypoglycemic (BG ≤ 60 mg/dL) animals were assigned to dose (5% dextrose SC) or no-dose treatment groups. Nearly all (87%) mice became hypoglycemic; 2 of these mice died. No significant intergroup difference in grimace score or mortality was detected. Overall, our results demonstrate that hypoglycemia is a frequent adverse event after RYGB in mice and that subcutaneous injection of dextrose is a safe and effective way to manage hypoglycemia. Further studies are necessary to optimize the intervention threshold and optimal dosage; regardless, we recommend glycemic management after RYGB surgery in mice. |
| Databáze: | OpenAIRE |
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