Translational Repression in the Arginine System of Escherichia coli
| Autor: | William L. McLellan, Henry J. Vogel |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Genetics
Microbial Arginine Auxotrophy Ornithine transcarbamylase Biology medicine.disease_cause Amidohydrolases Ornithine Carbamoyltransferase Transcription (biology) Genes Regulator Escherichia coli medicine RNA Messenger Psychological repression chemistry.chemical_classification Multidisciplinary Molecular biology Enzyme Biochemistry chemistry Genetic Code Mutation Biological Sciences: Biochemistry Rifampin |
| Zdroj: | Proceedings of the National Academy of Sciences. 67:1703-1709 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.67.4.1703 |
| Popis: | Translation of bacterial mRNA, divorced from transcription, has been obtained for enzymes of arginine synthesis; evidence has been acquired for repression by arginine at the level of translation. mRNAs for acetylornithinase and ornithine transcarbamylase were accumulated by arginine starvation of argR + and argR - arginine auxotrophs derived from Escherichia coli K12. Further transcription was inhibited with rifampicin or miracil D, and enzyme formation was measured in the presence of either an excess of, or a restricted supply of, arginine. For the argR + strain 961, little mRNA was found without starvation; for the argR - strain 977, a considerable amount of mRNA was demonstrated even without starvation. There was relatively little translation for the argR + strain, but not for the argR - strain, in the presence of excess arginine, apparently due to an accelerated degradation of mRNA in the argR + strain under repressive conditions. |
| Databáze: | OpenAIRE |
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