Characterization of the Murine μ Opioid Receptor Gene
| Autor: | Daniel L. Kaufman, Jide Tian, Douglas Newman, B. Anton, Yu-Rong Xia, Cindy Wen, Christopher J. Evans, Aldons J. Lusis, Daniel S. Lee, Karin Magendzo, Tuyet H. Tran, Duane E. Keith |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary medicine.drug_class Molecular Sequence Data Receptors Opioid mu CHO Cells Biology Biochemistry OGFr Mice Opioid receptor Cricetinae Enzyme-linked receptor medicine Animals Humans Amino Acid Sequence RNA Messenger Receptor Molecular Biology Peptide sequence In Situ Hybridization Brain Chemistry Mice Inbred BALB C Chinese hamster ovary cell Alternative splicing Chromosome Mapping Cell Biology Molecular biology Rats Mice Inbred C57BL Blotting Southern Opioid medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 270:15877-15883 |
| ISSN: | 0021-9258 |
| Popis: | The analgesic and addictive properties of morphine and other opioid drugs are thought to result from their interaction with mu opioid receptors. Using a delta opioid receptor cDNA as a probe, we have isolated a murine mu opioid receptor cDNA clone (mMOR). Stable expression of mMOR in Chinese hamster ovary cells conferred high binding affinity for mu receptor ligands including morphine and [D-Ala2,N-methyl-Phe4,Gly5-ol]-enkephalin and low affinity for delta and kappa preferring ligands. Treatment of these cell lines with morphine and other mu agonists inhibited forskolin-induced cAMP accumulation, demonstrating a functional coupling of mMOR to the inhibition of adenylate cyclase. The predicted amino acid sequence of mMOR shares approximately 55% overall amino acid identity with the delta receptor and approximately 97% identity with the recently reported rat mu opioid receptor. Expression of the mu receptor in mouse brain as revealed by in situ hybridization parallels the reported pattern of distribution of mu-selective ligand binding sites. Chromosomal localization (to mouse chromosome 10) and Southern analysis are consistent with a single mu opioid receptor gene in the mouse genome, suggesting that the various pharmacologically distinct forms of the mu receptor arise from alternative splicing, post-translational events, or from a highly divergent gene(s). |
| Databáze: | OpenAIRE |
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