Peritoneal macrophages are important for the early containment of Enterococcus faecium peritonitis in mice
| Autor: | Marc J. M. Bonten, Nico van Rooijen, Joris J. T. H. Roelofs, Ida A. J. Giebelen, Tom van der Poll, Masja Leendertse, Rob J. L. Willems |
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| Přispěvatelé: | Medical Microbiology and Infection Prevention, Center of Experimental and Molecular Medicine, ACS - Amsterdam Cardiovascular Sciences, AII - Amsterdam institute for Infection and Immunity, Pathology, Infectious diseases, Molecular cell biology and Immunology, CCA - Immuno-pathogenesis |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Chemokine
medicine.medical_treatment Phagocytosis Immunology Intraperitoneal injection Enterococcus faecium Peritonitis Microbiology Peritoneal cavity Mice In vivo medicine Animals Molecular Biology Gram-Positive Bacterial Infections biology business.industry Cell Biology biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Liposomes biology.protein Macrophages Peritoneal Cytokines Female Clodronic Acid business Ex vivo |
| Zdroj: | Innate immunity, 15(1), 3-12. SAGE Publications Ltd Innate Immunity, 15(1), 3-12. SAGE Publications Ltd Leendertse, M, Willems, R J L, Giebelen, I A J, Roelofs, J J T H, van Rooijen, N, Bonten, M J M & van der Poll, T 2009, ' Peritoneal macrophages are important for the early containment of Enterococcus faecium peritonitis in mice ', Innate Immunity, vol. 15, no. 1, pp. 3-12 . https://doi.org/10.1177/1753425908100238 |
| ISSN: | 1753-4259 |
| Popis: | The increasing incidence of infections with multi-drug resistant Enterococcus faecium necessitates studies to increase knowledge on the pathogenesis of these infections. In this study, the contribution of peritoneal macrophages during E. faecium peritonitis was investigated. In an ex vivo setting, peritoneal macrophages harvested from C57BL/6 mice were responsive to, and able to phagocytose and kill, E. faecium. In vivo, peritoneal macrophages were depleted by intraperitoneal injection of clodronate-encapsulated liposomes, prior to inducing E. faecium peritonitis. Depletion of resident peritoneal macrophages caused a clear delay in peritoneal clearance of E. faecium with increased systemic dissemination. Mice depleted of peritoneal macrophages were able to recruit macrophages and neutrophils to the peritoneal cavity after infection, comparable to control mice. Furthermore, increased levels of peritoneal cytokines and chemokines were found in mice depleted of peritoneal macrophages. This study indicates that peritoneal macrophages are important in the early containment of E. faecium peritonitis and for the regulation of the inflammatory response. |
| Databáze: | OpenAIRE |
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