Rab11 Is Associated with Transferrin-Containing Recycling Compartments in K562 Cells
| Autor: | Marianne Wessling-Resnick, Elizabeth Ramm, Nikki M. Riley, David Spiro, James R. Goldenring, Elizabeth G. Green |
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| Rok vydání: | 1997 |
| Předmět: |
Biophysics
Biology Polymerase Chain Reaction Biochemistry Exocytosis GTP-Binding Proteins Cell polarity Tumor Cells Cultured Humans Cloning Molecular Molecular Biology chemistry.chemical_classification Messenger RNA Cell Cycle Transferrin Cell Polarity Cell Biology Cell cycle Molecular biology Cell Compartmentation Membrane protein chemistry rab GTP-Binding Proteins Leukemia Erythroblastic Acute Rab K562 cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 239:612-616 |
| ISSN: | 0006-291X |
| Popis: | 3'RACE PCR was used to survey Rab transcripts synthesized by the human hematopoietic K562 cell line. Among the identified GTP-binding proteins, Rab11 was discovered. This result was unexpected since Rab11 previously had been found associated with polarized and secretory cells. Rab11 mRNA was abundant compared to that for other Rabs in K562 cells; protein levels represented 0.05-0.1% of total membrane protein. Localization of Rab11 using confocal immunofluoresence microscopy revealed extensive overlap with transferrin in recycling and/or exocytic compartments and suggests that Rab11 in non-polarized and non-secretory cells may play a role in the trafficking and recycling of internalized proteins. |
| Databáze: | OpenAIRE |
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