Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy
| Autor: | Ana Rita F. Pacheco, Beatriz D. Cardoso, Ana Pires, André M. Pereira, João P. Araújo, Violeta M. Carvalho, Raquel O. Rodrigues, Paulo J. G. Coutinho, Teresa Castelo-Grande, Paulo A. Augusto, Domingos Barbosa, Rui A. Lima, Senhorinha F. C. F. Teixeira, Ana Rita O. Rodrigues, Elisabete M. S. Castanheira |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
combined cancer therapy
Science & Technology Saúde de qualidade shape anisotropic nanoparticles Ciências Naturais::Ciências Físicas Engenharia e Tecnologia::Nanotecnologia General Chemical Engineering Ciências Médicas::Biotecnologia Médica General Materials Science pH-sensitive magnetoliposomes drug release superparamagnetism |
| Zdroj: | Nanomaterials; Volume 13; Issue 6; Pages: 1051 |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano13061051 |
| Popis: | Late diagnosis and systemic toxicity associated with conventional treatments make oncological therapy significantly difficult. In this context, nanomedicine emerges as a new approach in the prevention, diagnosis and treatment of cancer. In this work, pH-sensitive solid magnetoliposomes (SMLs) were developed for controlled release of the chemotherapeutic drug doxorubicin (DOX). Shape anisotropic magnetic nanoparticles of magnesium ferrite with partial substitution by calcium (Mg0.75Ca0.25Fe2O4) were synthesized, with and without calcination, and their structural, morphological and magnetic properties were investigated. Their superparamagnetic properties were evaluated and heating capabilities proven, either by exposure to an alternating magnetic field (AMF) (magnetic hyperthermia) or by irradiation with near-infrared (NIR) light (photothermia). The Mg0.75Ca0.25Fe2O4 calcined nanoparticles were selected to integrate the SMLs, surrounded by a lipid bilayer of DOPE:Ch:CHEMS (45:45:10). DOX was encapsulated in the nanosystems with an efficiency above 98%. DOX release assays showed a much more efficient release of the drug at pH = 5 compared to the release kinetics at physiological pH. By subjecting tumor cells to DOX-loaded SMLs, cell viability was significantly reduced, confirming that they can release the encapsulated drug. These results point to the development of efficient pH-sensitive nanocarriers, suitable for a synergistic action in cancer therapy with magnetic targeting, stimulus-controlled drug delivery and dual hyperthermia (magnetic and plasmonic) therapy. This work was funded by the Portuguese Foundation for Science and Technology (FCT) within the framework of the Strategic Funding of Research Units UIDB/04650/2020 (CF-UM-UP), UIDB/00319/2020 (ALGORITMI), UIDB/04077/2020 (MEtRICs), UIDB/04436/2020 (CMEMS) and UIDB/00511/2020 (LEPABE). FCT, POCI, FEDER and NORTE2020 are acknowledged for funding through research projects PTDC/QUI-QFI/28020/2017, EXPL/EMD-EMD/0650/2021, PTDC/EEI-EEE/2846/2021 and NORTE-01-0145-FEDER-000054. MINECO (Spain) is acknowledged for project MAT2016-76824-C3-2-R. B.D.C. and V.M.C. acknowledge FCT for PhD grants SFRH/BD/141936/2018 (B.D.C.) and UI/BD/151028/2021 (V.M.C.). R.O.R. thanks FCT for contract 2020.03975.CEECIND. |
| Databáze: | OpenAIRE |
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