Novel electrochemical biosensor for serotonin detection based on gold nanorattles decorated reduced graphene oxide in biological fluids and in vitro model
| Autor: | Amit Jaiswal, Keshav Bhardwaj, Buddhadev Purohit, Kuldeep Mahato, Pranjal Chandra |
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| Rok vydání: | 2019 |
| Předmět: |
Serotonin
Materials science Biomedical Engineering Biophysics Oxide Nanotechnology 02 engineering and technology Biosensing Techniques Electrochemistry 01 natural sciences law.invention Nanocomposites chemistry.chemical_compound law Limit of Detection Humans Detection limit Nanocomposite Graphene 010401 analytical chemistry General Medicine Electrochemical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Colloidal gold Graphite Gold Selected area diffraction 0210 nano-technology Biosensor Oxidation-Reduction Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 142 |
| ISSN: | 1873-4235 |
| Popis: | Abnormal level of serotonin (ST) in body fluids is related to various clinical conditions including behavioral and psychotic disorders; hence its fast detection in clinically relevant ranges have tremendous importance in medical science. In view of this, we have developed a novel biosensor for ST detection using Au-nanorattles (AuNRTs)- reduced graphene oxide (rGO) nanocomposite coated on to the gold nanoparticles (AuNPs) deposited glassy carbon electrode (GCE). The nanocomposite/sensor probe was characterized using UV–Vis, TEM, SAED, EDX, AFM, and electrochemical techniques including LSV and EIS. Thereafter, the suitability of fabricated GCE/AuNPs/AuNRTs-rGO-Naf sensor probe was applied for ST determination which showed a linear dynamic range (LDR) of 3 × 10−6 - 1 × 10−3 M and the detection limit (DL) of 3.87 (±0.02) ×10−7 (RSD |
| Databáze: | OpenAIRE |
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