Deltamethrin Resistance Mechanisms in Aedes aegypti Populations from Three French Overseas Territories Worldwide

Autor: Isabelle Dusfour, Clare Strode, Carlos Robello, Pilar Zorrilla, Romain Girod, Jean Issaly, Laurent Guillaumot, Amandine Guidez
Přispěvatelé: Unité d'entomologie médicale, Vectopôle Amazonien Emile Abonnenc [Cayenne, Guyane française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Molecular Biology / Biología Molecular [Montevideo], Institut Pasteur de Montevideo, Facultad de Medicina [Universidad de la Republica, Uruguay], Universidad de la República [Montevideo] (UCUR), Entomologie médicale [Nouméa, Nouvelle-Calédonie] (URE-EM), Institut Pasteur de Nouvelle-Calédonie, Edge Hill University, This work was funded by the Institut Pasteur funds through the ACIP-A-03-2010. This work has benefited from an 'Investissement d’Avenir' grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01). This study received a European commission 'REGPOT- CT-2011-285837-STRonGer'Grant within the FP7., ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), European Project: 285837,EC:FP7:REGPOT,FP7-REGPOT-2011-1,STRONGER(2011), Universidad de la República [Montevideo] (UDELAR)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Insecticides
Microarray
Voltage-Gated Sodium Channels
Insecticide Resistance
chemistry.chemical_compound
MESH: Mutant Proteins
0302 clinical medicine
Cytochrome P-450 Enzyme System
Aedes
Pyrethrins
MESH: Animals
Guadeloupe
Genetics
0303 health sciences
biology
Ecology
lcsh:Public aspects of medicine
MESH: Aedes
MESH: Nitriles
3. Good health
French Guiana
Infectious Diseases
MESH: Cytochrome P-450 Enzyme System
Female
DNA microarray
Research Article
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
030231 tropical medicine
Aedes aegypti
03 medical and health sciences
MESH: Gene Expression Profiling
MESH: Pyrethrins
New Caledonia
Nitriles
parasitic diseases
MESH: French Guiana
Animals
MESH: Guadeloupe
MESH: Insecticide Resistance
MESH: Voltage-Gated Sodium Channels
Gene
030304 developmental biology
Microarray analysis techniques
Gene Expression Profiling
Public Health
Environmental and Occupational Health
Cytochrome P450
lcsh:RA1-1270
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
biology.organism_classification
Microarray Analysis
MESH: New Caledonia
MESH: Insecticides
Gene expression profiling
MESH: Microarray Analysis
Deltamethrin
chemistry
biology.protein
Mutant Proteins
MESH: Female
Zdroj: PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (11), pp.e0004226. ⟨10.1371/journal.pntd.0004226⟩
PLoS Neglected Tropical Diseases, 2015, 9 (11), pp.e0004226. ⟨10.1371/journal.pntd.0004226⟩
PLoS Neglected Tropical Diseases, Vol 9, Iss 11, p e0004226 (2015)
ISSN: 1935-2727
1935-2735
DOI: 10.1371/journal.pntd.0004226⟩
Popis: Background Aedes aegypti is a cosmopolite mosquito, vector of arboviruses. The worldwide studies of its insecticide resistance have demonstrated a strong loss of susceptibility to pyrethroids, the major class of insecticide used for vector control. French overseas territories such as French Guiana (South America), Guadeloupe islands (Lesser Antilles) as well as New Caledonia (Pacific Ocean), have encountered such resistance. Methodology/Principal Findings We initiated a research program on the pyrethroid resistance in French Guiana, Guadeloupe and New Caledonia. Aedes aegypti populations were tested for their deltamethrin resistance level then screened by an improved microarray developed to specifically study metabolic resistance mechanisms. Cytochrome P450 genes were implicated in conferring resistance. CYP6BB2, CYP6M11, CYP6N12, CYP9J9, CYP9J10 and CCE3 genes were upregulated in the resistant populations and were common to other populations at a regional scale. The implication of these genes in resistance phenomenon is therefore strongly suggested. Other genes from detoxification pathways were also differentially regulated. Screening for target site mutations on the voltage-gated sodium channel gene demonstrated the presence of I1016 and C1534. Conclusion /significance This study highlighted the presence of a common set of differentially up-regulated detoxifying genes, mainly cytochrome P450 genes in all three populations. GUA and GUY populations shared a higher number of those genes compared to CAL. Two kdr mutations well known to be associated to pyrethroid resistance were also detected in those two populations but not in CAL. Different selective pressures and genetic backgrounds can explain such differences. These results are also compared with those obtained from other parts of the world and are discussed in the context of integrative research on vector competence.
Author Summary Aedes aegypti is vector of Dengue, Chikungunya and Zika viruses, all causing emerging or re-emerging diseases worldwide. Fighting these diseases relies on the control of the vector. Therefore, insecticides have been extensively used worldwide, resulting in the development of insecticide resistance. In the French overseas territories, resistance to pyrethroids has been monitored for many years with high levels in the South American French territories. We then investigated the mechanisms underlying this resistance in populations from French Guiana, Guadeloupe and New Caledonia. Transcription levels of detoxification genes were measured and alongside screening for target site mutations. Upregulation of cytochrome P450 genes and carboxylesterases were observed in all three populations. Mutations related to pyrethroid resistance in position 1016 and 1534 of the voltage-gated sodium channel gene were also observed. French Guiana and Guadeloupe populations presented a closer profile of resistance mechanisms whereas the New Caledonia population had a more restricted profile. Such differences can be explained by different vector control practices, regional insecticide uses and genetic backgrounds. These results are also compared with others obtained from other parts of the world and are discussed with the perspective of integrative research on vector competence.
Databáze: OpenAIRE
Externí odkaz: