Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
| Autor: | Guozheng Zhang, Jun-Qiang Jia, Chao Jin, Yun-Gang Jiang, Chu-Yan Wang, Zhong-Zheng Gui, Xijie Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
1-Deoxynojirimycin
Reishi Microorganism lcsh:QR1-502 Mineralogy Microbiology lcsh:Microbiology chemistry.chemical_compound Technology Pharmaceutical Response surface methodology Food science Enzyme Inhibitors microorganisms fermentation Chromatography High Pressure Liquid Ganoderma lucidum DNJ Production Single factor Potassium nitrate Hydrogen-Ion Concentration Culture Media Plant Leaves mulberry chemistry Food Microbiology Fermentation Morus Research Paper Biotechnology Mulberry leaf |
| Zdroj: | Brazilian Journal of Microbiology, Vol 45, Iss 2, Pp 721-729 (2014) Brazilian Journal of Microbiology v.45 n.2 2014 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 45, Issue: 2, Pages: 721-729, Published: JUN 2014 |
| ISSN: | 1678-4405 |
| Popis: | DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf. |
| Databáze: | OpenAIRE |
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