Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on proliferation and apoptosis of gingival epithelial cells
| Autor: | Ya-Qin Zhu, Long Jiang, Jun-jun Zhao, Xi-ping Feng |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Dose dependence
Gingiva Apoptosis Bacterial counts Flow cytometry Microbiology 03 medical and health sciences 0302 clinical medicine Lactobacillus acidophilus medicine Humans 030212 general & internal medicine Porphyromonas gingivalis Cell Proliferation Probiotic therapy biology medicine.diagnostic_test business.industry Mtt method food and beverages Epithelial Cells General Medicine biology.organism_classification Coculture Techniques Cytoprotection 030220 oncology & carcinogenesis business |
| Zdroj: | Advances in medical sciences. 64(1) |
| ISSN: | 1898-4002 |
| Popis: | Purpose This study aimed to evaluate the possible antagonistic effects of Lactobacillus acidophilus on Porphyromonas gingivalis, and detect inhibition of Lactobacillus acidophilus on Porphyromonas gingivalis when they are co-cultured with human gingival epithelial cells. Materials and methods Human gingival epithelial cells were co-cultured with Lactobacillus acidophilus and Porphyromonas gingivalis alone or together. The amount of Porphyromonas gingivalis adhering to or invading the epithelial cells were determined by bacterial counts. The cellular proliferation was assayed by the MTT method. Apoptosis was detected by flow cytometry with apoptosis detection kit. Results On one hand, Lactobacillus acidophilus reduced the inhibitory effect of Porphyromonas gingivalis on the human gingival epithelial cells proliferation in a dose dependent manner. On the other hand, Porphyromonas gingivalis induced significant apoptosis on human gingival epithelial cells, and Lactobacillus acidophilus inhibited this apoptosis-inducing effect of Porphyromonas gingivalis in a dose dependent manner. Conclusions Porphyromonas gingivalis inhibits the proliferation and induces the apoptosis of human gingival epithelial cells. Lactobacillus acidophilus could attenuate this effect in a dose-dependent manner, and it thus reduces the destruction from pathogens. Lactobacillus acidophilus could be an effective candidate for probiotic therapy in periodontal diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect