Solid phase synthesis of thymosin β9
| Autor: | R. B. Merrifield, N. Chandramouli, K. K. Bhargava, Mukund J. Modak, G. S. Incefy |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Oligopeptide Hydrocortisone Thymosin Peptide Biological activity Thymus Gland Biochemistry Mice Inbred C57BL Thymosin beta-4 Mice Structure-Activity Relationship Solid-phase synthesis chemistry Terminal deoxynucleotidyl transferase DNA Nucleotidylexotransferase Animals Indicators and Reagents Beta (finance) hormones hormone substitutes and hormone antagonists |
| Zdroj: | International Journal of Peptide and Protein Research. 28:536-541 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1986.tb03289.x |
| Popis: | Thymosin beta 9, a 41 residue thymic polypeptide, has been synthesized by a solid phase method. A modification of the low HF method was used to deprotect and cleave the peptide from the resin. Thymosin beta 9 was then obtained in analytically pure form by a one-step purification procedure in 32% yield. The activity of thymosin beta 9 in the terminal deoxynucleotidyl transferase assay was greater than calf thymus fraction 5, but comparable to thymosin beta 4. In contrast to thymosin alpha 1, neither beta 4 nor beta 9 was active in the rosette inhibition assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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