Dietary soy protein improves adipose tissue dysfunction by modulating parameters related with oxidative stress in dyslipidemic insulin-resistant rats

Autor: Paola Illesca, Yolanda B. Lombardo, Dante Selenscig, Silvina M. Alvarez, María Sofía Giménez, María del Rosario Ferreira, María Eugenia D'Alessandro
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
ANTIOXIDANT DEFENSES
Antioxidant
medicine.medical_treatment
Glutathione reductase
Ciencias de la Salud
Adipose tissue
medicine.disease_cause
Antioxidants
Protein Carbonylation
chemistry.chemical_compound
0302 clinical medicine
Dietary Sucrose
Adipocyte
Adipocytes
Insulin
OXIDATIVE STRESS
Soy protein
Adiposity
Epididymis
chemistry.chemical_classification
Glutathione peroxidase
Organ Size
General Medicine
ADIPOSE TISSUE
Adipose Tissue
Soybean Proteins
purl.org/becyt/ford/3 [https]
Dietary Proteins
SOY PROTEIN
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
NF-E2-Related Factor 2
VISCERAL ADIPOSITY
030209 endocrinology & metabolism
Biology
Thiobarbituric Acid Reactive Substances
purl.org/becyt/ford/3.3 [https]
03 medical and health sciences
Internal medicine
medicine
Animals
RNA
Messenger
Rats
Wistar
Dyslipidemias
HIGH SUCROSE DIET
Pharmacology
Glutathione Peroxidase
Salud Ocupacional
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Body Weight
Glutathione
Oxidative Stress
Glucose
030104 developmental biology
Endocrinology
chemistry
Energy Metabolism
Biomarkers
Oxidative stress
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2017.01.153
Popis: The present study investigates the benefits of the dietary intake of soy protein on adipose tissue dysfunction in a rat model that mimics several aspects of the human metabolic syndrome. Wistar rats were fed a sucrose-rich diet (SRD) for 4 months. After that, half of the animals continued with SRD until month 8 while in the other half, casein protein was replaced by isolated soy protein for 4 months (SRD-S). A reference group consumed a control diet all the time. In adipose tissue we determined: i) the activities of antioxidant enzymes, gene expression of Mn-superoxide dismutase (SOD) and glutathione peroxidase (GPx), and glutathione redox state ii) the activity of xanthine oxidase (XO), ROS levels and the gene expression of NAD(P)H oxidase iii) the expression of the nuclear factor erythroid-2 related factor-2 (Nrf2). Besides, adiposity visceral index, insulin sensitivity, and tumor necrosis factor-α (TNF-α) in plasma were determined. Compared with the SRD-fed rats, the animals fed a SRD-S showed: activity normalization of SOD and glutathione reductase, improvement of mRNA SOD and normalization of mRNA GPx without changes in the expression of the Nrf2, and improvement of glutathione redox state. These results were accompanied by a normalization of XO activity and improvement of both the ROS production as well as TNF-α levels in plasma. Besides, adipocyte size distribution, adiposity visceral index and insulin sensitivity improved. The results suggest that soy protein can be a complementary nutrient for treating some signs of the metabolic syndrome. Fil: Illesca, Paola Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Selenscig, Dante Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Ferreira Cordoneda, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Gimenez, Maria Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Bolzon, Yolanda Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: D'alessandro, Maria Eugenia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Databáze: OpenAIRE
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