Cryo-Electron microscopy for the study of self-assembled poly(ionic liquid) nanoparticles and protein supramolecular structures
| Autor: | Guosong Chen, Jiayin Yuan, Zdravko Kochovski, Yan Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Nanostructure
Materials science Polymers and Plastics Cryo-electron microscopy Nanoparticle Single particle analysis macromolecular substances 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry Microscopy Materials Chemistry Sample preparation Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences 021001 nanoscience & nanotechnology chemistry Chemical engineering Transmission electron microscopy ddc:540 Ionic liquid Institut für Chemie Inhouse research on structure dynamics and function of matter 0210 nano-technology |
| Zdroj: | Colloid and Polymer Science. 298:707-717 |
| ISSN: | 1435-1536 0303-402X |
| DOI: | 10.1007/s00396-020-04657-w |
| Popis: | Cryo-electron microscopy (cryo-EM) is a powerful structure determination technique that is well-suited to the study of protein and polymer self-assembly in solution. In contrast to conventional transmission electron microscopy (TEM) sample preparation, which often times involves drying and staining, the frozen-hydrated sample preparation allows the specimens to be kept and imaged in a state closest to their native one. Here, we give a short overview of the basic principles of Cryo-EM and review our results on applying it to the study of different protein and polymer self-assembled nanostructures. More specifically, we show how we have applied cryo-electron tomography (cryo-ET) to visualize the internal morphology of self-assembled poly(ionic liquid) nanoparticles and cryo-EM single particle analysis (SPA) to determine the three-dimensional (3D) structures of artificial protein microtubules. |
| Databáze: | OpenAIRE |
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