Characterization of an emergent high-risk KPC-producing Klebsiella pneumoniae lineage causing a fatal wound infection after spine surgery
| Autor: | Mayara Gil de Castro Santos, Priscila Lamb Wink, Humberlânia de Sousa Duarte, Eliane de Oliveira Ferreira, Gabriela Bergiante Kraychete, Renata Cristina Picão, Daiana de Lima-Morales, Rossiane C. Vommaro, Luana Boff, Carla Ormund Gonçalves Ximenes Lima, Vinícius Magno da Rocha |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Tetracycline Klebsiella pneumoniae Clone (cell biology) Virulence Biology Microbiology Plasmid Fatal Outcome Drug Resistance Multiple Bacterial Genetics medicine Humans Surgical Wound Infection Molecular Biology Gene Ecology Evolution Behavior and Systematics Aged Biofilm biology.organism_classification Spine Klebsiella Infections Infectious Diseases Wound Infection Female Bacteria medicine.drug |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 96 |
| ISSN: | 1567-7257 |
| Popis: | Surgical site infections in instrumented posterior lumbar interbody fusion surgery are normally due to gram-positive bacteria, but gram-negative bacteria can cause infections in cases involving lower lumbar interventions as its closer to the perianal area. Here we report an uncommon fatal wound infection caused by a multidrug-resistant Klebsiella pneumoniae after an elective spine surgery. In silico analysis revealed that LWI_ST16 belonged to ST16, an emergent international clone notable for its increased virulence potential. We also observed that this strain carried a conjugative IncF plasmid encoding resistance genes to beta-lactams (blaKPC-2 and blaOXA-1), tetracycline (tetA), aminoglycosides and fluoroquinolones (aac(6')-Ib-cr). The carbapenemase encoding gene blaKPC-2 was located on a Tn4401e transposon previously characterized to increase blaKPC expression. LWI_ST16 is a strong biofilm producer on polystyrene and capable of forming tower-like structures on a titanium device like the one inserted in the patient's spine. Our findings strengthen the valuable contribution of continuous surveillance of multidrug-resistant and high-risk K. pneumoniae clones to avoid unfavourable clinical outcomes. |
| Databáze: | OpenAIRE |
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