Hybridization of DNA targets to glass-tethered oligonucleotide probes
| Autor: | Lin Meng, Saralinda L. Turner, Dat D. Dao, Wanda G. Beattie, Rajender S. Varma, Kenneth L. Beattie |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Cystic Fibrosis Transmembrane Conductance Regulator Bioengineering Computational biology Biology Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry DNA sequencing Polyethylene Glycols chemistry.chemical_compound Sequencing by hybridization Molecular beacon Molecular Biology Base Sequence Oligonucleotide Hybridization probe Nucleic acid sequence Chromosome Mapping Nucleic Acid Hybridization Sequence Analysis DNA Molecular biology chemistry Mutation Glass DNA microarray Oligonucleotide Probes Sequence Alignment DNA Biotechnology |
| Zdroj: | Molecular Biotechnology. 4:213-225 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/bf02779015 |
| Popis: | Hybridization of nucleic acids to surface-tethered oligonucleotide probes has numerous potential applications in genome mapping and DNA sequence analysis. In this article, we describe a simple standard protocol for routine preparation of terminal amine-derivatized 9-mer oligonucleotide arrays on ordinary microscope slides and hybridization conditions with DNA target strands of up to several hundred bases in length with good discrimination against mismatches. Additional linker arms separating the glass surface from the probe sequence are not necessary. The technique described here offers a powerful tool for the detection of specific genetic mutations. |
| Databáze: | OpenAIRE |
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