Ectopic expression of Chrysanthemum CDM19 in Arabidopsis reveals a novel function in carpel development
| Autor: | Wenqian Zhao, Stephen Kironji Githeng’u, Lian Ding, Kunkun Zhao, Jiafu Jiang, Sumei Chen, Fadi Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gynoecium Chrysanthemum lcsh:Biotechnology Mutant Stamen Carpel development 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences lcsh:TP248.13-248.65 010608 biotechnology Arabidopsis MADS box lcsh:QH301-705.5 biology Floral organ identity Chrysanthemum morifolium biology.organism_classification Cell biology 030104 developmental biology lcsh:Biology (General) CDM19 Petal Ectopic expression Disc florets Silique Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Vol 45, Iss, Pp 10-18 (2020) |
| ISSN: | 0717-3458 |
| DOI: | 10.1016/j.ejbt.2020.03.001 |
| Popis: | Background APETALA3 (AP3) has significant roles in petal and stamen development in accordance with the classical ABC model. Results The AP3 homolog, CDM19, from Chrysanthemum morifolium cv. Jinba was cloned and sequenced. Sequence and phylogenetic analyses revealed that CDM19 is of DEF/AP3 lineage possessing the characteristic MIKC-type II structure. Expression analysis showed that CDM19 was transcribed in petals and stamens of ray and disc florets with weak expression in the carpels. Ectopic expression of CDM19 in Arabidopsis wild-type background altered carpel development resulting in multi-carpel siliques. CDM19 could only partially rescue the Arabidopsis ap3‐–3 mutant. Conclusions Our results suggest that CDM19 may partially be involved in petal and stamen development in addition to having novel function in carpel development. How to cite: Githeng’u SK, Ding L, Zhao K, et al. Ectopic expression of Chrysanthemum CDM19 in Arabidopsis reveals a novel function in carpel development. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.03.001 . |
| Databáze: | OpenAIRE |
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