Effects of individual and multiple fatty acids (palmitate, oleate and docosahaexenoic acid) on cell viability and lipid metabolism in LO2 human liver cells
| Autor: | Guoxun Chen, Shaoxin Huang, Bin Gong, Chunhong Wang, L.B. Chen, Qun Shi, Jun Yu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.medical_specialty Docosahexaenoic Acids Apolipoprotein B Cell Survival Palmitates Gene Expression Biochemistry chemistry.chemical_compound Internal medicine Genetics medicine Humans Viability assay Liver X receptor Molecular Biology Cells Cultured Triglycerides Liver X Receptors Apolipoprotein C-I biology Triglyceride Fatty Acids Lipid metabolism Lipid Metabolism Orphan Nuclear Receptors Sterol Up-Regulation PPAR gamma Endocrinology Liver Oncology chemistry Apoptosis Hepatocytes biology.protein Molecular Medicine Sterol Regulatory Element Binding Protein 1 Intracellular Oleic Acid |
| Zdroj: | Molecular Medicine Reports. 10:3254-3260 |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | This study was designed to investigate the direct effects of fatty acids (FAs) on the cell viability and the expression levels of genes involved in lipid metabolism in LO2 human liver cells. Palmitate (PA), oleate (OA) and docosahaexenoic acid (DHA) were used to represent saturated, mono-unsaturated and polyunsaturated FAs, respectively. At concentrations of ≤3.2 µg/ml, treatment with single FAs increased the viability of the LO2 cells. At FA concentrations of >3.2 µg/ml, cell viability following OA treatment was increased, but PA or DHA treatment at these concentrations reduced cell viability. Administration of mixtures of these FAs in three ratios (PA:OA:DHA = 1:2:1, 1:1:1 and 1:1:2, respectively) increased the cell viability compared with the control group. The intracellular triglyceride (TG) levels following all types of treatment were significantly increased and the accumulation of TGs was markedly increased with high doses of DHA. In addition, peroxisome proliferator-activated receptor-γ was significantly upregulated in all groups, with the exception of the 1:1:1 group at 3.2 µg/ml and the 1:1:2 group at 12.8 µg/ml. The expression levels of sterol regulatory-element binding protein‑1c, liver X receptor α and apolipoprotein C‑I were significantly reduced in all groups with the exception of the DHA‑treated group and the 1:2:1 groups at 3.2 and 12.8 µg/ml. In conclusion, these results indicate that the type, concentration and mixture ratios of FAs are all important in determining the cell viability and lipid metabolism-related gene expression in LO2 hepatocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|