Best practice recommendations for the measurement and interpretation of salivary proinflammatory cytokines in biobehavioral research
| Autor: | T. Hand, Jenna L. Riis, Katrina R. Hamilton, Douglas A. Granger, Hedyeh Ahmadi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine Saliva medicine.medical_treatment Blood contamination Interleukin-1beta Immunology Oral health Saliva sample Biospecimen Collection Article Proinflammatory cytokine 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine medicine Humans Tumor Necrosis Factor-alpha Endocrine and Autonomic Systems business.industry 030104 developmental biology Cytokine Cytokines Sample collection business 030217 neurology & neurosurgery |
| Zdroj: | Brain Behav Immun |
| ISSN: | 0889-1591 |
| DOI: | 10.1016/j.bbi.2020.09.009 |
| Popis: | Despite the integration of salivary inflammatory cytokines into research across the biobehavioral, psychological, clinical, and health-related disciplines, there is little guidance regarding the biospecimen collection, handling, and storage practices that maximize the quality and validity of salivary cytokine data. Furthermore, associations between salivary cytokines and measures related to oral health are rarely assessed and accounted for in studies outside the oral health fields. To address these gaps, we examine the sensitivity of salivary interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α) to changes in saliva sample collection technique and cold chain management procedures. Using subsets of saliva samples collected from 150 healthy adults, we measure salivary IL-1β, IL-6, IL-8, TNF-α, and other oral health-related indices (i.e., blood contamination [transferrin], and salivary matrixmallotprotienase-8). In addition to examining changes in cytokine levels associated with sample collection technique and cold chain management procedures, we assess relations between cytokine concentrations and levels of other oral health-related measures. We found that IL-1β, IL-6, and IL-8 were more robust to changes in sample collection and cold chain management procedures than TNF-α, and all cytokines were positively associated with other oral health-related measures. Based on our findings, we recommend analyte-specific guidance for measuring and interpreting salivary cytokine concentrations. |
| Databáze: | OpenAIRE |
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