Immature CD4+CD8+ thymocytes do not polarize lipid rafts in response to TCR-mediated signals
| Autor: | Jennifer A. Punt, Josh F. Baker, Peter J. R. Ebert |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
CD4-Positive T-Lymphocytes
T cell Morpholines Immunology Receptors Antigen T-Cell chemical and pharmacologic phenomena Stimulation Thymus Gland Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Mice Phosphatidylinositol 3-Kinases Membrane Microdomains medicine Immunology and Allergy Animals Enzyme Inhibitors Receptor Lipid raft Cytoskeleton Phosphoinositide-3 Kinase Inhibitors T-cell receptor CD28 Cell Polarity hemic and immune systems Cell Differentiation Actin cytoskeleton Actins Cell biology Enzyme Activation Mice Inbred C57BL medicine.anatomical_structure Chromones lipids (amino acids peptides and proteins) CD8 Signal Transduction |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 165(10) |
| ISSN: | 0022-1767 |
| Popis: | TCR-mediated stimulation induces activation and proliferation of mature T cells. When accompanied by signals through the costimulatory receptor CD28, TCR signals also result in the recruitment of cholesterol- and glycosphingolipid-rich membrane microdomains (lipid rafts), which are known to contain several molecules important for T cell signaling. Interestingly, immature CD4+CD8+ thymocytes respond to TCR/CD28 costimulation not by proliferating, but by dying. In this study, we report that, although CD4+CD8+ thymocytes polarize their actin cytoskeleton, they fail to recruit lipid rafts to the site of TCR/CD28 costimulation. We show that coupling of lipid raft mobilization to cytoskeletal reorganization can be mediated by phosphoinositide 3-kinase, and discuss the relevance of these findings to the interpretation of TCR signals by immature vs mature T cells. |
| Databáze: | OpenAIRE |
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