Prevalence and genotype distribution of rotaviruses in children with gastroenteritis in Rize province
| Autor: | Sumeyra Acar, Zekiye Bakkaloglu, Ayşegül Çopur Çiçek, Serdar Özkasap, Kadri Kanber, Riza Durmaz, Sadan Hacisalihoglu, Selim Dereci, Yücehan Albayrak |
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| Přispěvatelé: | RTEÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Dereci, Selim, Çiçek, Ayşegül Çopur, Özkasap, Serdar, Hacısalihoğlu, Şadan, Albayrak, Yücehan, Kanber, Kadri |
| Rok vydání: | 2015 |
| Předmět: |
Male
Rotavirus Genotyping medicine.medical_specialty Genotype Turkey Biology medicine.disease_cause Virus Rotavirus Infections children Molecular microbiology Rotaviruses Multiplex polymerase chain reaction Epidemiology medicine Prevalence Humans Children Feces Retrospective Studies lcsh:R5-920 Virology Gastroenteritis genotyping Child Preschool Female lcsh:Medicine (General) Research Article |
| Zdroj: | Bosnian Journal of Basic Medical Sciences, Vol 15, Iss 3 (2015) |
| ISSN: | 1840-4812 |
| Popis: | Bakkaloglu, Zekiye/0000-0001-9137-016X WOS: 000361745100005 PubMed: 26295292 Determination of the distribution of rotavirus genotypes is essential for understanding the epidemiology of this virus responsible for nearly half a million of deaths in patients with gastroenteritis worldwide. in the present study, we aimed to genotype the rotavirus strains isolated from diarrheal stool samples in children under 5 years old. A total of 1297 fecal samples were collected, and rotavirus antigen was detected in 73 of these samples. Antigen-positive samples were transferred to the Public Health Agency of Turkey, Molecular Microbiology Research Laboratory, and were tested for determination of genotypes G and P using semi-nested multiplex polymerase chain reaction method performed with consensus- and genotype-specific primers. Twelve specimens were found to be negative for rotavirus in genotyping method. All the positive-strains were in G1-4, G8-9, P(4), P(8), and P(9) genotypes. the most frequent GP genotype combinations were found to be G9P(8) in 21 strains (34.4%), G2P(4) in 14 strains (23.0%), and G1P(8) in 12 strains (19.7%). We found 10 distinct genotypes amongst a total of 61 strains. Among the strains isolated and genotyped in our study, 90.2% (55/61) and 67.2% (41/61) have already been included in the two existing commercial vaccines. in conclusion, these findings implicate the necessity of development of region-specific vaccines after evaluation of the local genotype distribution. Further studies on the large number of rotavirus strains would contribute to this process. |
| Databáze: | OpenAIRE |
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