Determination of mephenoxalone in human plasma sample by high-performance liquid chromatography–fluorescence detection
| Autor: | Kuang Yang Hsu, Yow Shieng Uang, I. Kei Chen, Li Hsuan Wang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Detection limit
Chromatography Muscle Relaxants Central Chemistry Analytical chemistry Reproducibility of Results General Chemistry Sensitivity and Specificity High-performance liquid chromatography Fluorescence Fluorescence spectroscopy Standard curve Absorbance Spectrometry Fluorescence Reference Values medicine Humans Mephenoxalone Oxazoles Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 759:91-97 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(01)00216-x |
| Popis: | A simple and sensitive high-performance liquid chromatographic method involving fluorescence detection was developed for the determination of mephenoxalone in human plasma. A Cosmosil 5C18-MS column (250 mm x 4.6 mm I.D., 5 microm) was used as stationary phase and the mobile phase consisted of water-acetic acid-acetonitrile (200:1:300) at a flow-rate of 1.0 ml/min. The fluorescence absorbance was monitored at 280 nm for excitation wavelength and 310 nm for emission wavelength. Temperature control was kept at 40 degrees C for the column. The limit of quantitation achieved was 10 ng/ml, and the standard curve was found to be linear in the concentration range of 10-10,000 ng/ml. Under these analytical conditions, a sufficient mephenoxalone plasma concentration profile could be obtained for pharmacokinetic study. |
| Databáze: | OpenAIRE |
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