TACE is required for fetal murine cardiac development and modeling
| Autor: | Jianping Sun, Hui Chen, David Warburton, Roberta G. Williams, Sue Buckley, Jingsong Zhao, Wei Shi, Kathryn D. Anderson |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
MAP Kinase Signaling System Cardiomyocyte Biology ADAM17 Protein Heart development 03 medical and health sciences Mice 0302 clinical medicine Internal medicine medicine Animals Receptor Molecular Biology 030304 developmental biology Mice Knockout 0303 health sciences Metalloproteinase TACE Cell growth Myocardium Metalloendopeptidases Heart Cell Biology Sheddase Cell biology ErbB Receptors ADAM Proteins Endocrinology Ectodomain 030220 oncology & carcinogenesis Knockout mouse Mutation Neuregulin Mitogen-Activated Protein Kinases Developmental Biology |
| Zdroj: | Developmental Biology. 261(2):371-380 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/s0012-1606(03)00315-4 |
| Popis: | Tumor necrosis factor-alpha converting enzyme (TACE) is a membrane-anchored, Zn-dependent metalloprotease, which belongs to the ADAM (a disintegrin and metalloprotease) family. TACE functions as a membrane sheddase to release the ectodomain portions of many transmembrane proteins, including the precursors of TNFalpha, TGFalpha, several other cytokines, as well as the receptors for TNFalpha, and neuregulin (ErbB4). Mice with TACE(DeltaZn/DeltaZn) null mutation die at birth with phenotypic changes, including failure of eyelid fusion, hair and skin defects, and abnormalities of lung development. Abnormal fetal heart development was not previously described. Herein, we report that TACE(DeltaZn/DeltaZn) null mutant mice by late gestation exhibit markedly enlarged fetal hearts with increased myocardial trabeculation and reduced cell compaction, mimicking the pathological changes of noncompaction of ventricular myocardium. In addition, larger cardiomyocyte cell size and increased cell proliferation were observed in ventricles of TACE(DeltaZn/DeltaZn) knockout mouse hearts. At the molecular level, reduced expression of epidermal growth factor receptor, attenuated protein cleavage of ErbB4, and changes in MAPK activation were also detected in TACE(DeltaZn/DeltaZn) knockout heart tissues. The data suggest that TACE-mediated cell surface protein ectodomain shedding plays an essential and a novel regulatory role during cardiac development and modeling. |
| Databáze: | OpenAIRE |
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