beta-(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen
| Autor: | Branca M. A. Silva, Alexandra Abrunheiro, Teresa Gonçalves, Jorge Anjos, Neil A. R. Gow, Chantal Fernandes, Célia Quintas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Antifungal Agents beta-Glucans Molecular Sequence Data Mutation Missense Microbial Sensitivity Tests Fungus Microbiology Cell wall Echinocandins Lipopeptides chemistry.chemical_compound Caspofungin Point Mutation DNA Fungal Phylogeny chemistry.chemical_classification Sequence Homology Amino Acid biology Nucleic acid sequence Alternaria Sequence Analysis DNA General Medicine Pathogenic fungus biology.organism_classification Infectious Diseases Enzyme Amino Acid Substitution chemistry Glucosyltransferases Drechslera |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | The fungal cell wall polymer beta-(1,3)-D-glucan is synthesized by the enzyme beta-(1,3)D-glucan synthase that is a complex composed of at least two proteins, Rho1p and Fks1p. Here, we report the nucleotide sequence of a single FKS gene and of the regulatory unit, RHO1 from the dematiaceous pathogenic fungus Alternaria infectoria. The predicted AiFks and AiRho share, respectively, 93% and 100% identity with that of Drechslera tritici-repentis. We also report that the sensitivity to caspofungin of eight different A. infectoria clinical strains is similar, with a MIC > 32 mu g/ml and a MEC of 1 mu g/ml, except for one strain which had a MEC of 1.4 mu g/ml. This same strain exhibited one substitution at the hot spot 2, S1405A, compatible with less susceptible phenotypes, with the other seven strains having no mutations in either hot spot 1 or 2. The relative quantification of the expression of AiFKS and of AiRHO demonstrated a decrease in response to an exposure to caspofungin at 0.5 mu g/ml. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|