Cross-Reactivity of Secondary Antibodies against African Rodents and Application for Sero-Surveillance
| Autor: | Ichiro Nakamura, Chihiro Sugimoto, Shintaro Kobayashi, Akihiro Ishii, Rie Isozumi, Ayato Takada, Kumiko Yoshimatsu, Bernard M. Hang’ombe, Yuka Thomas, Aaron S. Mweene, Yasuko Orba, Jiro Arikawa, Hirofumi Sawa |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Rodent
Population Zambia Enzyme-Linked Immunosorbent Assay Rodentia Biology Antibodies Viral Immunoglobulin G Serology Rodent Diseases Species Specificity biology.animal Animals Fluorescent Antibody Technique Indirect education Phylogeny Hantavirus education.field_of_study General Veterinary Murinae biology.organism_classification Antibodies Bacterial Virology Primary and secondary antibodies biology.protein Hantavirus Infection |
| Zdroj: | Journal of Veterinary Medical Science. 75:819-825 |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | A total of 466 rodents were captured in the Republic of Zambia from 2006 to 2010. Based on morphological observations and phylogenetic analyses of mitochondrial gene sequences, rodents were divided into 10 groups consisting of 39 Rattus rodents, 263 multimammate rats, 18 other Murinae rodents, 95 gerbils, 11 pouched mice, 1 giant-pouched rat, 38 fat mice and 1 dormouse. Rodent antibodies except that from Rattus were examined for their cross-reactivity to commercially available antibody detection reagents. Anti-mouse immunoglobulin G (IgG) was most cross-reactive to heterologous antibodies including multimammate rat, gerbil, pouched mouse and fat mouse. Thus, anti-mouse IgG would be a useful detection tool in serological examination of the Zambian rodent population. Preliminary sero-surveillance for plague, leptospirosis and hantavirus infection was performed by ELISA. |
| Databáze: | OpenAIRE |
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