Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona
| Autor: | Ievgen Shulov, Andrey S. Klymchenko, Vitaly I. Kalchenko, Roman V. Rodik, Andreas Reisch, Youri Arntz |
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| Rok vydání: | 2016 |
| Předmět: |
Azides
Dispersity Nanoparticle 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Micelle Catalysis Article Polyethylene Glycols chemistry.chemical_compound Amphiphile Calixarene Humans Cyanine Particle Size Micelles Fluorescent Dyes 010405 organic chemistry Chemistry Optical Imaging Proteins General Medicine General Chemistry Carbocyanines 021001 nanoscience & nanotechnology Fluorescence 3. Good health 0104 chemical sciences Förster resonance energy transfer Cross-Linking Reagents Nanoparticles Click Chemistry Calixarenes 0210 nano-technology HeLa Cells |
| Zdroj: | Angewandte Chemie International Edition |
| ISSN: | 1521-3773 |
| Popis: | The key challenge in the field of fluorescent nanoparticles (NPs) for biological applications is to achieve superior brightness for sizes equivalent to single proteins (3–7 nm). We propose a concept of shell-cross-linked fluorescent micelles, in which PEGylated cyanine 3 and 5 bis-azides form a covalently attached corona on micelles of amphiphilic calixarene bearing four alkyne groups. The fluorescence quantum yield of the obtained monodisperse NPs, with a size of 7 nm, is a function of viscosity and reached up to 15 % in glycerol. In the on-state they are circa 2-fold brighter than quantum dots (QD-585), which makes them the smallest PEGylated organic NPs of this high brightness. FRET between cyanine 3 and 5 cross-linkers at the surface of NPs suggests their integrity in physiological media, organic solvents, and living cells, in which the NPs rapidly internalize, showing excellent imaging contrast. Calixarene micelles with a cyanine corona constitute a new platform for the development of protein-sized ultrabright fluorescent NPs. |
| Databáze: | OpenAIRE |
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