Genipin crosslinked chitosan/gelatin blends for biomedical applications
| Autor: | Ettore Pulieri, Arti Ahluwalia, Giovanni Vozzi, Valeria Chiono, Paolo Giusti, Gianluca Ciardelli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Materials science
food.ingredient Tertiary amine Surface Properties Biomedical Engineering Biophysics Biocompatible Materials Bioengineering macromolecular substances Gelatin Biomaterials Chitosan Contact angle Mice chemistry.chemical_compound food Cell Line Tumor Tensile Strength Polymer chemistry Cell Adhesion medicine Animals Iridoids Cell Proliferation Neurons technology industry and agriculture Adhesion Fibroblasts Thermogravimetry chemistry Iridoid Glycosides NIH 3T3 Cells Genipin Swelling medicine.symptom Nuclear chemistry |
| Popis: | Blends between chitosan (CS) and gelatin (G) with various compositions (CS/G 0/100 20/80, 40/60, 60/40 100/0 w/w) were produced, as candidate materials for biomedical applications. Different amounts of genipin (0.5 wt.% and 2.5 wt.%) were used to crosslink CS/G blends, promoting the formation of amide and tertiary amine bonds between the macromolecules and the crosslinker. The effects of composition and crosslinking on the physico-chemical properties of samples were evaluated by infrared analysis, thermogravimetry, contact angle measurements, dissolution and swelling tests. Mechanical properties of crosslinked samples were also determined through stress-strain and creep tests: samples stiffness increased with increasing the crosslinker amount and the CS content. Blend composition affected mouse fibroblasts adhesion and proliferation on substrates, depending on the crosslinker amount. Finally, crosslinked CS/G blends containing 80 wt.% G were found to support neuroblastoma cells adhesion and proliferation which made them promising candidates for uses in the field of nerve regeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|