Examining the effect of wound cleansing on the microbiome of venous stasis ulcers
Autor: | Kianna M Blount, Craig Howser, Kristina K Zudock, Abdulnaser Alkhalil, Lauren T. Moffatt, David K. Karig, Jeffrey W. Shupp, Collin M. Timm, Amanda Ernlund |
---|---|
Rok vydání: | 2021 |
Předmět: |
Wound Healing
Standard of care integumentary system business.industry Microbiota Venous Stasis Ulcers Dermatology medicine.disease Bandages Venous leg ulcer Dressing change Varicose Ulcer Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S medicine Humans Surgery Microbiome Wound cleansing business Wound treatment |
Zdroj: | Wound Repair and Regeneration. 29:766-776 |
ISSN: | 1524-475X 1067-1927 |
Popis: | Common treatment for venous leg wounds includes topical wound dressings with compression. At each dressing change, wounds are debrided and washed; however, the effect of the washing procedure on the wound microbiome has not been studied. We hypothesized that wound washing may alter the wound microbiome. To characterize microbiome changes with respect to wound washing, swabs from 11 patients with chronic wounds were sampled before and after washing, and patient microbiomes were characterized using 16S rRNA sequencing and culturing. Microbiomes across patient samples prior to washing were typically polymicrobial but varied in the number and type of bacterial genera present. Proteus and Pseudomonas were the dominant genera in the study. We found that washing does not consistently change microbiome diversity but does cause consistent changes in microbiome composition. Specifically, washing caused a decrease in the relative abundance of the most highly represented genera in each patient cluster. The finding that venous leg ulcer wound washing, a standard of care therapy, can induce changes in the wound microbiome is novel and could be potentially informative for future guided therapy strategies. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |