Cryopreservation of human sperm in a lecithin-supplemented freezing medium
| Autor: | Spencer Land, Vida C. Acosta, Rajasingam S. Jeyendran, Carolyn B. Coulam |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
food.ingredient Cell Survival Cell Culture Techniques Serum albumin Lecithin Cryopreservation Andrology chemistry.chemical_compound fluids and secretions food Phosphatidylcholine Yolk Lecithins Humans Food science Cells Cultured Sperm motility Sperm Count biology Dimethyl sulfoxide Obstetrics and Gynecology Spermatozoa Sperm Culture Media Reproductive Medicine chemistry Sperm Motility biology.protein Semen Preservation |
| Zdroj: | Fertility and Sterility. 90:1263-1265 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2007.10.068 |
| Popis: | The use of egg yolk and serum albumin as additive diluents for human sperm cryopreservation is routine. But because both diluents are of animal origin, they potentially may introduce microbial agents to the sample. To reduce the risk of contamination, the cryoprotective property of phospholipids extracted from lecithin was evaluated and found to be effective when supplemented with dimethyl sulfoxide and glycerol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|