Aqueous dynamic and histological findings after deep sclerectomy with collagen implant in an animal model
| Autor: | André Mermoud, T Delarive, Tarek Shaarawy, S Rossier, A Rossier, Emilie Ravinet |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Intraocular pressure genetic structures Hemodynamics Haematoxylin Clinical Science - Extended Reports Aqueous Humor Cellular and Molecular Neuroscience chemistry.chemical_compound Animal model Ophthalmology medicine Animals Glaucoma Drainage Implants Intraocular Pressure Eosin business.industry Editorials Histology Glaucoma eye diseases Sensory Systems Surgery Sclera medicine.anatomical_structure chemistry Models Animal Sclerostomy Female sense organs Implant Collagen Rabbits business |
| Zdroj: | The British journal of ophthalmology. 87(11) |
| ISSN: | 0007-1161 |
| Popis: | The use of an animal model to study the aqueous dynamic and the histological findings after deep sclerectomy with (DSCI) and without collagen implant.Deep sclerectomy was performed on rabbits' eyes. Eyes were randomly assigned to receive collagen implants. Measurements of intraocular pressure (IOP) and aqueous outflow facility using the constant pressure method through cannulation of the anterior chamber were performed. The system was filled with BSS and cationised ferritin. Histological assessment of the operative site was performed. Sections were stained with haematoxylin and eosin and with Prussian blue. Aqueous drainage vessels were identified by the reaction between ferritin and Prussian blue. All eyes were coded so that the investigator was blind to the type of surgery until the evaluation was completed.A significant decrease in IOP (p0.05) was observed during the first 6 weeks after DSCI (mean IOP was 13.07 (2.95) mm Hg preoperatively and 9.08 (2.25) mm Hg at 6 weeks); DS without collagen implant revealed a significant decrease in IOP at weeks 4 and 8 after surgery (mean IOP 12.57 (3.52) mm Hg preoperatively, 9.45 (3.38) mm Hg at 4 weeks, and 9.22 (3.39) mm Hg at 8 weeks). Outflow facility was significantly increased throughout the 9 months of follow up in both DSCI and DS groups (p0.05). The preoperative outflow facility (OF) was 0.15 (0.02) micro l/min/mm Hg. At 9 months, OF was 0.52 (0.28) microl/min/mm Hg and 0.46 (0.07) micro l/min/mm Hg for DSCI and DS respectively. Light microscopy studies showed the appearance of new aqueous drainage vessels in the sclera adjacent to the dissection site in DSCI and DS and the apparition of spindle cells lining the collagen implant in DSCI after 2 months.A significant IOP decrease was observed during the first weeks after DSCI and DS. DS with or without collagen implant provided a significant increase in outflow facility throughout the 9 months of follow up. This might be partly explained by new drainage vessels in the sclera surrounding the operated site. Microscopic studies revealed the appearance of spindle cells lining the collagen implant in DSCI after 2 months. |
| Databáze: | OpenAIRE |
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