Superoxide Dismutase Prevents SARS-CoV-2-Induced Plasma Cell Apoptosis and Stabilizes Specific Antibody Induction
| Autor: | Li-Hua Mo, Xiang-Qian Luo, Ke Ma, Jian-Bo Shao, Guo-Hao Zhang, Zhi-Gang Liu, Da-Bo Liu, Huan-Ping Zhang, Ping-Chang Yang |
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| Rok vydání: | 2022 |
| Předmět: |
Male
Aging COVID-19 Vaccines Article Subject viruses Plasma Cells Immunization Secondary Apoptosis Antibodies Viral Biochemistry Mice Immunogenicity Vaccine Superoxide Dismutase-1 Adjuvants Immunologic Animals QH573-671 SARS-CoV-2 Alcoholic Beverages Vaccination COVID-19 virus diseases Cell Biology General Medicine Janus Kinase 2 respiratory system biochemical phenomena metabolism and nutrition Antibodies Neutralizing Specific Pathogen-Free Organisms respiratory tract diseases Mice Inbred C57BL Oxidative Stress STAT1 Transcription Factor Spike Glycoprotein Coronavirus Alum Compounds Angiotensin-Converting Enzyme 2 Cytology Reactive Oxygen Species Heat-Shock Response Research Article Signal Transduction |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2022 (2022) |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2022/5397733 |
| Popis: | The infection of coronavirus disease (COVID-19) seriously threatens human life. It is urgent to generate effective and safe specific antibodies (Abs) against the pathogenic elements of COVID-19. Mice were immunized with SARS-CoV-2 spike protein antigens: S ectodomain-1 (CoV, in short) mixed in Alum adjuvant for 2 times and boosted with CoV weekly for 6 times. A portion of mice were treated with Maotai liquor (MTL, in short) or/and heat stress (HS) together with CoV boosting. We observed that the anti-CoV Ab was successfully induced in mice that received the CoV/Alum immunization for 2 times. However, upon boosting with CoV, the CoV Ab production diminished progressively; spleen CoV Ab-producing plasma cell counts reduced, in which substantial CoV-specific Ab-producing plasma cells (sPC) were apoptotic. Apparent oxidative stress signs were observed in sPCs; the results were reproduced by exposing sPCs to CoV in the culture. The presence of MTL or/and HS prevented the CoV-induced oxidative stress in sPCs and promoted and stabilized the CoV Ab production in mice in re-exposure to CoV. In summary, CoV/Alum immunization can successfully induce CoV Ab production in mice that declines upon reexposure to CoV. Concurrent administration of MTL/HS stabilizes and promotes the CoV Ab production in mice. |
| Databáze: | OpenAIRE |
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