Prolyl endopeptidase-degraded low immunoreactive wheat flour attenuates immune responses in Caco-2 intestinal cells and gluten-sensitized BALB/c mice
| Autor: | Vijay S. Shinde, Nawneet K. Kurrey, B.V. Mohan Kumar, P. Prabhasankar, M. Vijaykrishnaraj |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Glutens
Flour Wheat flour Toxicology Immunoglobulin E BALB/c 03 medical and health sciences Mice 0404 agricultural biotechnology Prolyl endopeptidase Splenocyte medicine Animals Humans Triticum 030304 developmental biology Plant Proteins chemistry.chemical_classification 0303 health sciences Mice Inbred BALB C biology Chemistry Hydrolysis Serine Endopeptidases food and beverages 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science Gluten Molecular biology Small intestine Celiac Disease medicine.anatomical_structure biology.protein Female Caco-2 Cells Inflammation Mediators Gliadin Prolyl Oligopeptidases Food Science medicine.drug |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 129 |
| ISSN: | 1873-6351 |
| Popis: | Targeted degrading Aspergillus niger-derived prolyl endopeptidase (AN-PEP) is promising in gluten hydrolysis because it specifically cleaves the proline-rich sites in gluten. The current study aims to understand the safety aspects of AN-PEP hydrolysed low immunoreactive wheat flours by testing immune responses using cell line and animal models. In the AN-PEP hydrolysed wheat flour (AN-PEP HWF) gliadin extract, there was no increase in the levels of zonulin-1 (Zo-1) and pro-inflammatory cytokines (IL-6 and IL-8) but a significant increase was noted in the control wheat flour (CWF) gliadin-treated Caco-2 cells. The Zo-1 localization in Caco-2 cells was significantly noted in the reacted positive fluorescence cells that were treated with the control wheat flour. Further, a safety evaluation of HWF was carried out in gluten-sensitized BALB/c mice. Mouse anti-gliadin (IgG, IgA and IgE) antibodies were significantly generated in the CWF treated animals rather than the AN-PEP HWF groups. The serum pro-inflammatory (IL-1β, IL-4, IL-6, IL-15, TNF-α and IFN-γ) markers were observed in significant levels in CWF challenged mice and a similar trend was observed in ex-vivo splenocyte cells. A small intestine histopathological sectioning revealed that there are no abnormalities or structural changes in AN-PEP HWF challenged mice. |
| Databáze: | OpenAIRE |
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