Xk-Related Protein 8 and CED-8 Promote Phosphatidylserine Exposure in Apoptotic Cells
| Autor: | Jun Suzuki, Daniel P. Denning, H. Robert Horvitz, Shigekazu Nagata, Eiichi Imanishi |
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| Rok vydání: | 2013 |
| Předmět: |
Phospholipid scramblase
Recombinant Fusion Proteins Phagocytosis Molecular Sequence Data Apoptosis Phosphatidylserines Cell Line Mice chemistry.chemical_compound Phospholipid scrambling Cell Line Tumor Animals Humans Amino Acid Sequence Caenorhabditis elegans Proteins Caenorhabditis elegans Caspase Mice Knockout Multidisciplinary biology Macrophages Cell Membrane Membrane Proteins Phosphatidylserine biology.organism_classification Cell biology chemistry Caspases DNA methylation biology.protein Calcium CpG Islands Apoptosis Regulatory Proteins |
| Popis: | A classic feature of apoptotic cells is the cell-surface exposure of phosphatidylserine (PtdSer) as an "eat me" signal for engulfment. We show that the Xk-family protein Xkr8 mediates PtdSer exposure in response to apoptotic stimuli. Mouse Xkr8(-/-) cells or human cancer cells in which Xkr8 expression was repressed by hypermethylation failed to expose PtdSer during apoptosis and were inefficiently engulfed by phagocytes. Xkr8 was activated directly by caspases and required a caspase-3 cleavage site for its function. CED-8, the only Caenorhabditis elegans Xk-family homolog, also promoted apoptotic PtdSer exposure and cell-corpse engulfment. Thus, Xk-family proteins have evolutionarily conserved roles in promoting the phagocytosis of dying cells by altering the phospholipid distribution in the plasma membrane. アポトーシス時のリン脂質暴露に関与する因子の同定. 京都大学プレスリリース. 2013-07-12. |
| Databáze: | OpenAIRE |
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