Assessment of clinical significance of anti-Ge in an untransfused man
| Autor: | J. M. Watt, B. R. Wylie, H. A. Pearson, D. Bruce, Henry M. Kronenberg, V. L. Richards, D. Wilkie |
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| Rok vydání: | 1991 |
| Předmět: |
Adult
Male medicine.medical_specialty Immunology Gastroenterology Isoantibodies Internal medicine medicine Humans Immunology and Allergy Clinical significance New Guinea Isotopes of chromium biology Red Cell Monocyte Antibody-Dependent Cell Cytotoxicity Erythrocyte Aging Hematology Chromium Radioisotopes Red blood cell medicine.anatomical_structure Blood Group Antigens biology.protein Antibody Indirect Antiglobulin Test Complication |
| Zdroj: | Transfusion. 31:257-259 |
| ISSN: | 1537-2995 0041-1132 |
| DOI: | 10.1046/j.1537-2995.1991.31391165177.x |
| Popis: | A 19-year-old, untransfused Melanesian man from Papua New Guinea was admitted to the hospital for repair of an atrial septal defect. His serum contained an alloantibody that reacted strongly on the indirect antiglobulin test and was identified as anti-Ge. Gerbich-negative blood was transfused following urgent surgery. A 51Cr red cell survival study performed 2 weeks after surgery yielded zero survival of Gerbich-positive cells after 24 hours. A monocyte-driven, antibody-dependent, cell-mediated cytotoxicity assay performed on both pretransfusion and posttransfusion serum samples and on concentrated serum showed less than 1 percent specific lysis of Gerbich-positive cells. This did not correlate with the indication of clinical significance predicted by the 51Cr study. Red cell adherence and phagocytosis, not evident in a monocyte monolayer assay using native serum, were demonstrable in 16 percent of monocytes by the use of concentrated serum. |
| Databáze: | OpenAIRE |
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