Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis
Autor: | Liang-Hao Hu, Zhuan Liao, Fei Jiang, Jin-Huan Lin, Jun-Tao Ji, Ting-Ting Du, Zhao-Shen Li, Lei Xin, Dan Wang, Jun Gao |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Adult
Male 0301 basic medicine Oncology medicine.medical_specialty Disease Article 03 medical and health sciences Fibrosis Pancreatitis Chronic Internal medicine microRNA Cluster Analysis Humans Medicine RNA Messenger Pancreatitis chronic Oligonucleotide Array Sequence Analysis Multidisciplinary business.industry Case-control study medicine.disease body regions MicroRNAs Early Diagnosis 030104 developmental biology medicine.anatomical_structure ROC Curve Area Under Curve Case-Control Studies embryonic structures Pancreatitis Female DNA microarray business Pancreas Biomarkers |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep40019 |
Popis: | Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed miRNAs (DEmiRs) of CP patients from Gene Expression Omnibus (GEO), and the DEmiRs in plasma of early CP patients (n = 10) from clinic by miRNA microarrays. Expression levels of DEmiRs were further tested in clinical samples including early CP patients (n = 20), late CP patients (n = 20) and healthy controls (n = 18). The primary endpoints were area under curve (AUC) and expression levels of DEmiRs. Four DEmiRs (hsa-miR-320a-d) were obtained from GEO CP, meanwhile two (hsa-miR-221 and hsa-miR-130a) were identified as distinct biomarkers of early CP by miRNA microarrays. When applied on clinical serum samples, hsa-miR-320a-d were accurate in predicting late CP, while hsa-miR-221 and hsa-miR-130a were accurate in predicting early CP with AUC of 100.0% and 87.5%. Our study indicates that miRNA expression profile is different in early and late CP. Hsa-miR-221 and hsa-miR-130a are biomarkers of early CP, and the panel of the above 6 serum miRNAs has the potential to be applied clinically for early diagnosis of CP. |
Databáze: | OpenAIRE |
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