Revising the taxonomy of the Acinetobacter lwoffii group: The description of Acinetobacter pseudolwoffii sp. nov. and emended description of Acinetobacter lwoffii
| Autor: | Martina Maixnerova, Jaroslav Bzdil, Petra Španělová, Lenka Radolfová-Křížová, Petr Ježek, Alexandr Nemec, Dominique Clermont, Matěj Nemec |
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| Přispěvatelé: | Laboratory of Bacterial Genetics, National Institute of Public Health, Charles University [Prague] (CU), Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris], State Veterinary Institute Olomouc, Příbram Regional Hospital, The work was partially supported by MH CZDRO (National Institute of Public Health − NIPH, 75010330)., Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Core genome Applied Microbiology and Biotechnology Microbiology MESH: Bacterial Typing Techniques 03 medical and health sciences Phylogenetics MALDI-TOF MS Whole genome sequence Clade MESH: Phylogeny Ecology Evolution Behavior and Systematics Phylogeny 030304 developmental biology Genetics 0303 health sciences biology Acinetobacter Whole Genome Sequencing 030306 microbiology Carbon source assimilation DNA-Directed RNA Polymerases biology.organism_classification rpoB Bacterial Typing Techniques MESH: DNA-Directed RNA Polymerases Taxon Genus Acinetobacter MESH: Acinetobacter Genes Bacterial [SDE]Environmental Sciences Taxonomy (biology) MESH: Genes Bacterial Acinetobacter lwoffii MESH: Whole Genome Sequencing |
| Zdroj: | Systematic and Applied Microbiology Systematic and Applied Microbiology, Elsevier, 2019, 42 (2), pp.159-167. ⟨10.1016/j.syapm.2018.10.004⟩ Systematic and Applied Microbiology, 2019, 42 (2), pp.159-167. ⟨10.1016/j.syapm.2018.10.004⟩ |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/j.syapm.2018.10.004⟩ |
| Popis: | International audience; In 1986, Bouvet and Grimont delineated two related taxa of the genus Acinetobacter termed genospecies (GS) 8 and 9. They proposed the name Acinetobacter lwoffii for GS8, which included the supposed type strain (CIP 64.10). As the authenticity of CIP 64.10 was later questioned, this study aimed at reassessing the taxonomy of these genospecies. We investigated 52 strains of GS8 or GS9, including CIP 64.10 and the genuine type strain of A. lwoffii (NCTC 5866T). All strains were subjected to the genus-wide comparative analyses of MALDI-TOF whole-cell mass spectra, rpoB gene sequences and metabolic traits while whole-genome sequences were analysed for 16 strains. The strains were classified into two distinct groups corresponding to GS8 (n=15) and GS9 (n=37). CIP 64.10 fell within GS8 whereas NCTC 5866T belonged to GS9. Intraspecies ANIb values for the genomes of GS8 (n=6) and GS9 (n=10) were ≥96.1% and ≥95.4%, respectively, whereas the ANIb values between them were 86.8-88.6%. Based on core genome phylogeny, GS8 and GS9 formed a distinct clade within the genus, with two respective, strongly supported subclades. GS8 and GS9 were similar in physiological and catabolic properties but were separable by MALDI-TOF MS. We conclude that the name A. lwoffii pertains to GS9 and not to GS8 as originally assumed and that these groups represent two species. We propose the name Acinetobacter pseudolwoffii sp. nov. for GS8, with ANC 5044T (=CCM 8638T=CCUG 67963T=CIP 111642T) as the type strain, and provide the emended description of A. lwoffii. |
| Databáze: | OpenAIRE |
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